AEST Standard 24- cell tissue/cell culture techniques

AEST Standard 24- cell tissue/cell culture techniques What is often the major source of explants for tissue culture? - ANSWER-the meristematic ends of the plants like the stem tip or axillary bud What are the major benefits of plant tissue culture? - ANSWER-The ability to quickly produce mature plants, produce multiples of plants in the absence of seeds or pollinators, produce copies of plants with desirable traits, produce plants that otherwise have a low chance of germinating, mass propagate plants for commercial use, and produce disease free plants. Why are plant hormones often added to growth media in tissue culture? - ANSWER-To increase plant growth What can be used to sterilize the air and exposed work surfaces in a cell culture hood? - ANSWER-UV light Which direction should your covers or lids face when setting them down on the work surface? - ANSWER-Down, to prevent airborne contaminants from landing on the inside of them How should you sterilize your hands and work surface? - ANSWER-By wiping them down with 70% alcohol What is the purpose of a laminar airflow hood in a tissue culture facility? - ANSWER-To protect the tissue culture f