ASCP MB (MOLECULAR BIOLOGY ASCP) ASCP MB (Newest 2024 /2025) Extra -Practice 200 Questions With Verified Answers The Klenow fragment of E. coli DNA polymerase I is used: {Correct Ans: - to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? {Correct Ans: - gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visibl e with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. {Correct Ans: - Gel mobility shift assay Which probe is most often used to detect trisomy 21 by interphase FISH? {Correct Ans: - alpha satellite DNA probe _____ detect specific chromosomes and numbers of the specific chromosomes. {Correct Ans: - Alpha satellite DNA probe Which reagent generates a signal during the annealing stage of a quantitative real time PCR reaction? {Correct Ans: - molecular beacon Methylation of cytosine residues is involved in the alteration of gene expression in a number of cancers. Which sequencing technique is best suited to determine if methylation of cytosine residues has taken place in the promoter region of a given gene? {Correct Ans: - bisulfite sequencing _____ is a modified chain terminator method specifically designed to detect methylated nucleotides. {Correct Ans: - Bisulfite sequencing Which method is best suited to screen for mutations in a gene having multiple possib le mutations? {Correct Ans: - single strand conformational polymorphism (SSCP) _____ is a screening test in which the exact nature of a mutation does not need to be known in order to detect the presence of a mutation. {Correct Ans: - Single strand conform ational polymorphism (SSCP) ASCP MB (MOLECULAR BIOLOGY ASCP) What is the best method to rule out the possibility of genomic DNA giving a false -
positive result in an RT -PCR reaction? {Correct Ans: - use of upstream and downstream primers that span an exon -intron -exon region of the target *amplicaons generated from genomic DNA would be larger than the specific amplicon generated from a mature, spliced mRNA A common use for pulsed -field gel electrophoresis is: {Correct Ans: - epidemiological typing of bacterial strains. _____ after digest ion of bacterial chromosomes with restriction enzymes is used to determine similarity between bacterial isolates. {Correct Ans: - PFGE A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product associated with CML? a. 11;18 b. 14;18 c. 9;22 d. 9;14 {Correct Ans: - t(9;22) The presence of a distinct band along with the smear indicates at least 1% of the patient's B cells have a _____ of the Ig heavy chain. {Correct Ans: - monoclonal rearrangement A molecular -based test commonly used in testing donors and recipients prior to organ transplantation is: {Correct Ans: - sequence -specific PCR. _____ is commonly performed to determine polymorphisms in class I and class II HLA-DRB and HLA -DRQ genes. {Correct Ans: - Sequence -specific PC R DNA sequencing of hypervariable regions I and II is used to: {Correct Ans: - detect polymorphisms in human mitochondrial DNA. HV1 and HV2 are sequenced to determine _____. {Correct Ans: - mitochondrial inheritance. ASCP MB (MOLECULAR BIOLOGY ASCP) Repeated discrepancies between patie nt identifiers will result in _____ {Correct Ans: - the error being documented to the laboratory supervisor and the risk manager will conduct a root cause analysis. An index of precision is statistically known as the: {Correct Ans: - coefficient of variat ion. The _____ is the measure of relative random error expressed as a percentage. {Correct Ans: - coefficient of variation The term used to describe reproducibility is: {Correct Ans: - precision. _____ is the degree to which results of multiple analyses of the same specimen agree. {Correct Ans: - Precision The process by which an agency or organization uses predetermined standards to evaluate and recognize a program of study in an institution is called: {Correct Ans: - accreditation. Using a common labeling system for hazardous material identification such as HMIS or NFPA 704, which hazard is represented by: the top red quadrant? the right yellow quadrant? the lower white quadrant? the left blue quadrant? {Correct Ans: - flammability reactivity special reactivity health If the HMIS or NFPA 704 hazardous material identification system has a number 4 in the left blue quadrant, it represents a: {Correct Ans: - high health hazard
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