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Microbiology Lab Final Exam 2024, 223 Questions and Correct Answers.
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Microbiology Lab Final Exam 2024, 223 Questionsand Correct Answers.
agent that kills bacteria upon exposure
bacteriocidal
agent naturally produced by microorganisms
antibiotic
agent that slows the growth of bacteria
bacteriostatic
agent artificially synthesized or manipulated from other products
antimicrobial
targets DNA gyrase, preventing DNA replication within a cell
novobiocin
Disrupts cell wall stability, eventually leading to cell lysis
penicillin
binds with bacterial ribosomes to prevent cell growth and cell division
gentamicin
the mold that alexander fleming observed preventing bacterial growth was
penicillium notatum
since the discovery and use of antibiotics, we no longer need to worry about bacterial infections
false
penicillin works by
disrupting cell wall stability
novobiocin prevents the function of ______ in a cell
DNA gyrase
bacteriostatic antibiotics cause an inhibition of growth rather than cell lysis and death
true
gentamicin binds with bacterial _______
ribosomes
,the effective zone of inhibition is the same for all antibiotics
false
an antibiotic is a synthetic drug produced in a laboratory
false
______ is a genus that produces antibiotics
all of the above: Bacillys, Cephalosporium, and Streptomyces
penicillin is produced by a ____
mold
novobiocin is a bacteriostatic, narrow-spectrum antibiotic
true
gentamicin is synthesized by a ___
gram-positive bacteria
bacteriocidal agents kill bacteria by _____
cell wall lysis
resistant bacteria will exhibit ____ during a kirby-bauer diffusion test
no zone of inhibition
a spreader is used when inoculating a kirby-bauer diffusion plate to ___
create a uniform bacterial lawn
zones of inhibition should be measured after they exceed 25 mm
false
the diagram below illustrates resistance to drug ___ (nothing around A, little around B, large around C)
A
______ are asexually produced spores of the penicillum fungus
conidia
describes individual colonies on a plate's surface
colony forming unit
the number of individuals doubles each generation
exponential growth
cloudiness of a fluid caused by large numbers of particles/organisms
, turbidity
the measure of the light intensity absorbed by a sample
absorbance
asexual reproduction resulting in two daughter cells
binary fission
order the steps for performing a dilution series
1. determine the dilutions of the dilution tubes
2. determine which plate is the countable plate
3. calculate the concentration in the original tube
binary fission is a method of sexual reproduction where a single cell (parent cell) will split into four
cells (daughter cells)
false
plates with ____ colonies per plate are used to calculate CFUs
30-300
if the 1:100 plate contained 50 colonies, the 1:1000 plate contained 200 colonies, and the 1:10000
dilution plate contained 1000 colonies which plate would you use to determine the original culture
concentrations?
the 1:100 plate
in binary fission bacterial cells grow ___
exponentially
when one bacterial cell undergoes binary fission, there will be ___ cells present on the 5th generation
16
a viable plate count is a method of calculating the number of cells in a dilution by counting the
number of _____ on agar plates
colonies
performing a 1:8 dilution from the original sample 3 times would produce a dilution of
1:512
to create a series of dilutions, an identical volume of stock (undiluted) solution is added to each of the
dilution test tubes
false
plate _____ in the photo should be used for a viable plate count
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