(MB) ASCP Practice Exam Questions With Complete Solutions

(MB) ASCP Practice Exam Questions With Complete Solutions Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base - Answer Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: - Answer Thymine What genes would be screened in a breast cancer panel? - Answer HER2, ERBB2, BRCA1 Next Generation Sequencing uses: - Answer Short sequence reads Purines and pyrimidines differ from each other in that: - Answer Purines have two rings; pyrimidines have one ring The purines are: Cytosine and uracil Adenine and thymine Thymine and cytosine Adenine and guanine - Answer Adenine and guanine What is the rate of mutation per round of DNA replication? 1 in 1,000 base pairs 1 in 10,000 base pairs 1 in 1,000,000 base pairs 1 in 1,000,000,000 base pairs - Answer 1 in 1,000,000,000 base pairs The rate of DNA migration through an agarose gel during electrophoresis does not depend on which of the following factors? Net charge of the molecule Size of the molecule Shape of the molecule Nucleotide sequence of the molecule - Answer Nucleotide sequence of the molecule What are the phases in a qPCR Amplification Plot? Initiation, exponential, plateau Baseline, exponential, plateau Baseline, threshold, exponential, plateau Baseline, initiation, threshold, exponential, plateau - Answer Initiation, exponential, plateau Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'? 5'-GAC 3'-GAC 3'-CTG 5'-GTC - Answer 3'-GAC A patient with impaired judgment, personality changes, signs of abnormal body movements and depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates that patient has Huntington Disease, HD. Which of the following would be consistent with this diagnosis? 25 CAG repeats in the Huntington gene 85 CAG repeats in the Huntington gene 25 CGA repeats in the Huntington gene 85 CGA repeats in the Huntington gene - Answer 85 CAG repeats in the Huntington gene Which two HPV types are responsible for most cases of cervical cancer? 16 and 18 31 and 59 16 and 58 44 and 59 - Answer 16 and 18 Replication forks, known as origins of DNA replication, are created by this enzyme: Ligase Taq Polymerase Primase Helicase - Answer Helicase Mutation in what gene is associated with Fragile X syndrome? - Answer FMR1 Mantle cell lymphoma (MCL) is caused by what translocation? - Answer t(11;14) This polymerase is involved in "initiation of DNA replication and has primase activity": - Answer Pol α Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA on both leading and lagging strands of DNA: Klenow fragment of DNA polymerase Okazaki fragments Sanger fragments RNA fragments - Answer Okazaki fragments What gene is measured following treatment with imatinib (Gleevec