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Phlebotomy Exam Questions with 100% Correct Answers Verified | Updated 2024/2025

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Phlebotomy Exam Questions with 100% Correct Answers Verified | Updated 2024/2025 A condition in which the concentration of blood cells is increased in proportion to the plasma. Hemoconcentration The destruction of dissolution of red blood cells, with subsequent release of hemoglobin. Hemoly...

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  • May 6, 2024
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Phlebotomy Exam Questions with 100%
Correct Answers Verified | Updated
2024/2025
A condition in which the concentration of blood cells is increased in proportion to the plasma.
Hemoconcentration


The destruction of dissolution of red blood cells, with subsequent release of hemoglobin.
Hemolysis


The liquid portion of whole blood that contains active clotting agents.
Plasma


The liquid portion of the whole blood that remains after the blood has clotted.
Serum


A material that appears to be solid until subjected to a disturbance, such as centrifugation,
whereupon it becomes a liquid.
Thixotropic Gel


Venipuncture Equipment
double pointed safety needles, evacuated stopper tubes, needle holder, sharps container, syringes,
winged infusion sets ( butterfly needles ), tourniquet, marking pen, isopropyl alcohol swabs, gauze
pads, bandages, gloves.


Most common way to locate a vein before blood is drawn
Tourniquets and palpation


A plastic or glass sealed vacuum tube used to collect a blood specimen obtained through
venipuncture.
Evacuated Collection Tubes/ Vacutainer


Tube Additives
All tubes except the red-topped one contain an additive. Anticoagulants are added to prevent blood
from clotting, additive may be a powder or liquid visible in the tube or liquid sprayed inside the tube
and allowed to dry.


ADDITIVE AND FUNCTION-->SPS prevents blood from clotting and stabilizes bacterial growth
LABORATORY USE--> Blood and body fluid cultures
5ml <--OPTIMUM VOL
N/A<--MINIMUM volume
Yellow Tube


ADDITIVE AND FUNCTION--> None
LABORATORY USE--> Serum tests; chemistry studies, blood bank, serology.
10ml<-- MINIMUM VOLUME

, N/A<--OPT volume
Red Tube


ADDITIVE AND FUNCTION--> None, but contains silica particles to enhance clot formation
LABORATORY USE--> Serum test
10ml<--OPTIMUM VOLUME
N/A<--MINIMUM volume
Red-Gray (marbled) Tube/ Gold


ADDITIVE AND FUNCTION--> Sodium citrate; removes calcium to prevent blood from clotting
LABORATORY USE--> Coagulation testing
4.5ml <--MINIMUM/OPT VOLUME
Light blue Tube


ADDITIVE AND FUNCTION--> Heparin ( sodium/lithium/ammonium ); inhibits thrombin formation to
prevent clotting.
LABORATORY USE--> Chemistry test
10ml <--OPTIMUM VOL
3.5ml <--MINIMUM VOL
Green Tube


ADDITIVE AND FUNCTION--> Lithium heparin and gel for plasma separation
LABORATORY USE--> Plasma determinations in chemistry studies
2ml<--MINIMUM/OPT VOLUME
Green-gray (marbled) Tube/ Light Green


ADDITIVE AND FUNCTION--> Thrombin
LABORATORY USE--> Stat serum demonstrations in chemistry studies
2ml <--MINIMUM/OPT VOLUME
Yellow-gray (marbled) Tube/ Orange


ADDITIVE AND FUNCTION--> EDTA; removes calcium to prevent blood from clotting
LABORATORY USE--> Hematology tests
7ml <--OPTIMUM VOL
2ml <-- MINIMUM VOL
Lavender Tube


ADDITIVE AND FUNCTION --> Potassium oxalate and sodium fluoride; removes calcium to prevent
blood from clotting; fluoride inhibits glycolysis
LABORATORY USE --> Chemistry testing, especially glucose and alcohol levels
10ml <-- MINIMUM/OPT VOLUME
Gray Tube


ADDITIVE AND FUNCTION--> Sodium heparin (also sodium EDTA) inhibits thrombin formation to
prevent clotting
LABORATORY USE --> Chemistry trace elements
7ml <-- OPTIMUM VOL
Royal Blue Tube

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