Microbiology Lab Questions Correctly Solved.

Microbiology Lab Questions Correctly Solved. there are many types of staining tech used in microbiology. which of the following is not a commonly used staining - CORRECT ANSWER wright's stain - CORRECT ANSWER which of the following stains do not require heat fixation? - CORRECT ANSWER capsule stain The ___ stain provides critical information about peptidoglycan structure of the cell wall. - CORRECT ANSWER Gram Which of the following stains require heat fixation? - CORRECT ANSWER Endospore Gram Simple Acid-fast Gram stain - CORRECT ANSWER a stain that can differentiate between cells that have thick or thin layers of peptidoglycan in their cell walls Acid-Fast stain - CORRECT ANSWER a stain that would be appropriate for cells that contain mycolic acid in their cell walls. Capsule stain - CORRECT ANSWER a stain that enables you to effectively visualize a bacterium's glycocalyx Which of the following provides a quick and effective method used to visualize and determine bacterial morphology and arrangement? - CORRECT ANSWER Simple stain If you decolorize for more than 30 seconds, gram-positive organisms will most likely appear ____ in color. - CORRECT ANSWER pink What might happen if you omitted the safranin step while performing the Gram stain on the mixed culture of gram-positive and gram-negative cells? - CORRECT ANSWER Gram-negative organisms might not be visible if you omit the Gram's iodine step while preforming the Gram stain, gram-positive cells will most likely be ____ in color. - CORRECT ANSWER pink What outcome could be expected if you forgot the crystal violet step while performing a Gram stain? - CORRECT ANSWER Gram-positive and gram-negative organisms would stain pink Gram-negative cells appear __ after Gram staining whereas gram-positive cells appear __. - CORRECT ANSWER red, purple If you forget to use the decolorizer, which cell type(s) will appear purple? - CORRECT ANSWER Gram-positive and Gram-negative What is the primary dye used in the gram staining technique? - CORRECT ANSWER crystal violet What is used as the mordant in the gram staining technique? - CORRECT ANSWER Gram's iodine which of the following correctly describe how the Gram stain works to color different types of cells? - CORRECT ANSWER gram-positive cells retain the purple dye-mordant complex because of their thick layer of peptidoglycan. gram-negative cells are decolorized and stained by the pink/red secondary stain which of the following describes the correct sequence of the gram stain procedure? - CORRECT ANSWER heat-fixed slide; use of crystal violet, iodine, decolorizer, and safranin