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NEW BIO251 - EXAM 2 ACTUAL EXAM WITH CORRECT ANSWERS GRADE A+

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NEW BIO251 - EXAM 2 ACTUAL EXAM WITH CORRECT ANSWERS GRADE A+ 3 main process of DNA replication - -answer-- initiation - elongation - termination Central Dogma - -answer-DNA -> RNA -> Protein The process by which DNA is used to encode proteins what some of the characteristics of tra...

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  • June 4, 2024
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NEW BIO251 - EXAM 2 ACTUAL EXAM WITH
CORRECT ANSWERS GRADE A+
3 main process of DNA replication - -answer-- initiation
- elongation
- termination

Central Dogma - -answer-DNA -> RNA -> Protein

The process by which DNA is used to encode proteins

what some of the characteristics of transcription/RNA - -
answer-- Only specific regions are copies
- RNA strands are produced, which do not bind with the DNA
(exist as single strands)
- No proofreading

what are the 5 steps in transcription - -answer-Step 1: RNA
polymerase binds to the promoter (a regulatory region directly
upstream of the gene) and initiates DNA unraveling

Step 2 : RNA polymerase facilitates the assembly of free
ribonucleotides into chains of RNA, using the DNA as a template

Step 3: The RNA polymerase continues along the gene adding to
the RNA chain, which does not permanently pair with the DNA,
but exists as a single strand

Step 4: RNA synthesis continues until the RNA polymerase
encounters a specific region of DNA known as a terminator

,Step 5: Once the terminator is reached, the RNA polymerase
stalls and a rho termination protein facilitates the RNA
polymerase and RNA strand to disassociate from the DNA
The DNA is re-wound and the RNA is free to go on to the next
step

name for groups of 3 base pairs of the mRNA - -answer-codons

how many codons are non-coding or stop codons? - -answer-3
are non-coding or stop codons
only 61 of the 64 encode amino acids

which molecule can bind both RNA and amino acids - -answer-
T-RNA

group of three bases on a tRNA molecule that are
complementary to an mRNA codon - -answer-Anticodon

t-RNA is considered to be "charged" - -answer-when an amino
acid is bound to t-RNA

how many different t-RNA there are - -answer-64, one for each
codon


the study of how the heredity material (DNA and RNA) is
transferred. - -answer-genetics

all genetic material in a cell - -answer-genome

what is the sub division of genome - -answer-- chromosome
- plasmid

,what is the sub division of chromosomes - -answer-genes
(coding region)
non-coding region

what composes the DNA back bone - -answer-sugar
phosphate

which are the 4 nucleotides in DNA - -answer-Adenine
Thymine
Cytosine
Guanine

Uracil (only in RNA)

what is the difference between bacterial and Eukaryota
chromosomes - -answer-Most bacteria have only one circular
chromosome, whereas eukaryotes tend to have multiple linear
chromosomes

Why is DNA semi-conservative? - -answer-When DNA divides,
each copy receives one half of the original DNA and one half new
DNA.

enzyme(s) that uncoiled the DNA - -answer-topoisomerase and
gyrase, and begins at a region called the origin of replication

enzyme that unwinds and begins separating the DNA strands in
both directions - -answer-helicase

enzyme that stabilize the single stranded DNA - -answer-single
strand binding proteins

, what is required for the addition of nucleotides in DNA
replication? - -answer-a free 3' hydroxyl group

enzyme that adds an RNA primer to the strand being replicated -
-answer-primase

enzyme that makes sure the proper nucleotide is bound, and
connects it to the growing DNA strand - -answer-DNA
polymerase

DNA lagging strand - -answer-- DNA is synthesized in pieces
- multiple RNA primers are added
- assembled together by the enzymes DNA -polymerase and
ligase


the structures where protein synthesis (translation) occurs - -
answer-Ribosomes

which are the 2 microbial ribosome subunits - -answer-the 30S
and 50S, which come together to form a functional 70S ribosome

what does the "S" in ribosomal subunits stands for - -answer-
Svedberg units, which measures the rate of sedimentation
during centrifugation

what are the 3 functional domains of the large microbial
ribosome subunit - -answer-Aminoacyl site (A)
site of entry for tRNA's

Peptidyl (P) site
Site of peptide bond formation

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