BIOTECHNOLOGY PRINCIPLES AND PROCESSES 75
CHAPTER 11
B IOTECHNOLOGY : P RINCIPLES
AND PROCESSES
MULTIPLE-CHOICE QUESTIONS
1. Rising of dough is due to:
a. Multiplication of yeast
b. Production of CO2
c. Emulsification
d. Hydrolysis of wheat flour starch into sugars.
2. An enzyme catalysing the removal of nucleotides from the ends of
DNA is:
a. endonuclease
b. exonuclease
c. DNA ligase
d. Hind - II
3. The transfer of genetic material from one bacterium to another through
the mediation of a vector like virus is termed as:
a. Transduction
b. Conjugation
c. Transformation
d. Translation
4. Which of the given statement is correct in the context of observing DNA
separated by agarose gel electrophoresis?
a. DNA can be seen in visible light
b. DNA can be seen without staining in visible light
c. Ethidium bromide stained DNA can be seen in visible light
d. Ethidium bromide stained DNA can be seen under exposure to
UV light
, 76 BIOLOGY, EXEMPLAR PROBLEMS
5. 'Restriction' in Restriction enzyme refers to:
a. Cleaving of phosphodiester bond in DNA by the enzyme
b. Cutting of DNA at specific position only
c. Prevention of the multiplication of bacteriophage in bacteria
d. All of the above
6. A recombinant DNA molecule can be produced in the absence of the
following:
a. Restriction endonuclease
b. DNA ligase
c. DNA fragments
d. E.coli
7. In agarose gel electrophoresis, DNA molecules are separated on the basis
of their:
a. Charge only
b. Size only
c. Charge to size ratio
d. All of the above
8. The most important feature in a plasmid to be used as a vector is:
a. Origin of replication (ori)
b. Presence of a selectable marker
c. Presence of sites for restriction endonuclease
d. Its size
9. While isolating DNA from bacteria, which of the following enzymes is not
used?
a. Lysozyme
b. Ribonuclease
c. Deoxyribonuclease
d. Protease
10. Which of the following has popularised the PCR (polymerase chain
reactions)?
a. Easy availability of DNA template
b. Availability of synthetic primers
c. Availability of cheap deoxyribonucleotides
d. Availability of 'Thermostable' DNA polymerase
CHAPTER 11
B IOTECHNOLOGY : P RINCIPLES
AND PROCESSES
MULTIPLE-CHOICE QUESTIONS
1. Rising of dough is due to:
a. Multiplication of yeast
b. Production of CO2
c. Emulsification
d. Hydrolysis of wheat flour starch into sugars.
2. An enzyme catalysing the removal of nucleotides from the ends of
DNA is:
a. endonuclease
b. exonuclease
c. DNA ligase
d. Hind - II
3. The transfer of genetic material from one bacterium to another through
the mediation of a vector like virus is termed as:
a. Transduction
b. Conjugation
c. Transformation
d. Translation
4. Which of the given statement is correct in the context of observing DNA
separated by agarose gel electrophoresis?
a. DNA can be seen in visible light
b. DNA can be seen without staining in visible light
c. Ethidium bromide stained DNA can be seen in visible light
d. Ethidium bromide stained DNA can be seen under exposure to
UV light
, 76 BIOLOGY, EXEMPLAR PROBLEMS
5. 'Restriction' in Restriction enzyme refers to:
a. Cleaving of phosphodiester bond in DNA by the enzyme
b. Cutting of DNA at specific position only
c. Prevention of the multiplication of bacteriophage in bacteria
d. All of the above
6. A recombinant DNA molecule can be produced in the absence of the
following:
a. Restriction endonuclease
b. DNA ligase
c. DNA fragments
d. E.coli
7. In agarose gel electrophoresis, DNA molecules are separated on the basis
of their:
a. Charge only
b. Size only
c. Charge to size ratio
d. All of the above
8. The most important feature in a plasmid to be used as a vector is:
a. Origin of replication (ori)
b. Presence of a selectable marker
c. Presence of sites for restriction endonuclease
d. Its size
9. While isolating DNA from bacteria, which of the following enzymes is not
used?
a. Lysozyme
b. Ribonuclease
c. Deoxyribonuclease
d. Protease
10. Which of the following has popularised the PCR (polymerase chain
reactions)?
a. Easy availability of DNA template
b. Availability of synthetic primers
c. Availability of cheap deoxyribonucleotides
d. Availability of 'Thermostable' DNA polymerase
