1. Which of the following lamps provides a continuous
spectrum of radiant energy in the visible, near IR, and near
UV regions of the spectrum?
A. Tungsten-filament
B. Hydrogen
C. Deuterium
D. Mercury Vapor - ANSWER -A. Tungsten-filament
2. Which of the following isolates light within a narrow region
of the spectrum?
A. Photomultiplier tube
,B. Monochromator
C. Photovoltaic cell
D. Detector - ANSWER -B. Monochromator
3. Which of the following is not descriptive of a
photomultiplier tube?
A. Emits electrons proportionally to initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received - ANSWER -C. Cannot
be used with a chopper
4. Which of the following is false about a photomultiplier
tube?
A. Converts radiant energy (light) to electrical energy
(current)
B. Amplifies the current significantly
C. Has a very rapid response time
,D. Is composed of an iron plate and a layer of selenium -
ANSWER -D. Is composed of an iron plate and a layer of
selenium
5. Which type of photodetector employs a linear arrangement
that allows it to respond to a specific wavelength resulting in
complete UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array - ANSWER -D. Photodiode array
6. When performing spectrophotometer quality assurance
checks, what is the holmium oxide glass filter used to
assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
, D. Wavelength accuracy - ANSWER -D. Wavelength accuracy
7. In spectrophotometric analysis, what is the purpose of the
reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents - ANSWER -
D. Correct for color contribution of the
reagents
8. In regard to bichromatic analysis, which
of the following is false?
A. Absorbance is measured at the spectral absorbance peak
for a blank and the sample using the same wavelength.
B. Eliminates background interferences
C. Sample concentration determined from difference in two
measured absorbances
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