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Biosafety Levels (BSL 1-4) Microbiology Questions and Answers
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Biosafety Levels (BSL 1-4) Microbiology Questions and Answers
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Biosafety Levels (BSL 1-4) Microbiology Questions and AnswersBSL 1 - Health risk - ANS-Not known to cause disease in healthy humans
BSL 2 - Health risk - ANS-Associated with human disease in healthy but easily contained. Transmission through percutaneous, ingestion, mucous membrane.
BSL 3 - Health risk - ANS-Disease may have serious or lethal consequences
BSL 4 - Health risk - ANS-Agents with high risk of life threatening disease
BSL 1 - Practices - ANS-Standard open bench microbiology
BSL 2 - Practices - ANS-BSL-1 practice plus: Limited access, Biohazard warning signs, "Sharps" precautions, disposal of waste and surveillance.
BSL 3 - Practices - ANS-BSL-2 practice plus: Controlled access
BSL 4 - Practices - ANS-BSL-3 practice plus
BSL 1 - Primary Barriers - ANS-None required
BSL 2 - Primary Barriers - ANS-Class II Biosafety cabinet (BSC) and containment devices for aerosols or splashes (goggles, masks); lab coats and gloves
BSL 3 - Primary barriers - ANS-BSL-2 plus BSC use for all procedures
BSL 4 - Primary barriers - ANS-BSL-3 plus complete isolation
BSL 1 - Secondary Barriers - ANS-Open bench-sink; goggles and gloves for liquids
BSL 2 - Secondary Barriers - ANS-BSL-1 plus autoclave available
BSL 3 - Secondary Barriers - ANS-BSL-2 plus
BSL 4 - Secondary Barriers - ANS-BSL-3 plus
BSL 1 - Organisms - ANS-Many used
BSL 2 - Organisms - ANS-Some used, indicated as BSL-2. Staphyloccocus sp., Proteus
sp., Bacillus cereus, Pseudomonas, Enterococcus, Klebsiella, Chromobacterium, Salmonella, Morganella, Shigella
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