Mass spectrometry Quiz Questions Correctly Answers
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Course
Mass spectrometry
Institution
Mass Spectrometry
what is the purpose of an inlet for a mass spectrometer? - ️️the place where the sample is introduced to the mass spectrometer
what is the purpose of the source for a mass spectrometer? - ️️it converts neutral analytes into ions
what is the purpose of the analyzer for a mass spectrometer?...
what is the purpose of an inlet for a mass spectrometer? - ✔ ✔ the place where the sample is introduced to the mass spectrometer what is the purpose of the source for a mass spectrometer? - ✔ ✔ it converts neutral analytes into ions what is the purpose of the analyzer for a mass spectrometer? - ✔ ✔ it separates ions based on m/z (mass to charge ratio) what is the purpose of the dete ctor for a mass spectrometer? - ✔ ✔ it counts the number of ions reaching the detector what are the typical ion sources for GC? - ✔ ✔ electron impact ionization (EI) or chemical ionization (CI) what are the typical ion sources for LC? - ✔ ✔ electro -spray i onization (ESI) or Atmospheric pressure chemical ionization (APCI) or atmospheric pressure photoionization (APPI) compare in detail the difference between electron impact ionization and chemical ionization (hard vs. soft, ions produced, mass spectrum) - ✔ ✔ EI - hard ionization technique CI - soft ionization technique EI- produces high energy molecular ions positively charged radical molecular ions CI - produces less energy molecular ions forms [M + H]⁺ ions EI - mass spectrum - each peak corresponds to a fragment due to excess energy CI - no fragmentation, adduct ions are seen due to variety of chemical reactions taking place describe in detail electro -spray ionization (ESI) - ✔ ✔ - soft ionization technique
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