BACE test Questions and Answers

BACE test Questions and Answers A technician is assigned the task of inserting a gene, lanA, into a plasmid to ultimately be expressed as a therapeutic protein in E. coli. Label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. Transform E. coli with the plasmid containing lanA. Digest the amplified lanA PCR product and plasmid with appropriate restriction enzymes. Design primers to amplify the lanA sequence using polymerase chain reaction (PCR), and perform the PCR reaction. Use ligase to "paste" lanA into a plasmid. 4 2 1 3 After performing the PCR reaction with the primers she designed to amplify lanA, the same technician found that her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 kilobase pairs. What is the most likely reason there is an unexpected band in her PCR reaction? a. She added too many nucleotides b. She added too much polymerase c. She used an annealing temperature that was too low d. She used an annealing temperature that was too high c She used an annealing temperature that was too low In a biotechnology lab, tap water is best used for: a) Making buffers b) The first rinse when washing glassware c) The final rinse when washing glassware d) All of the above b The first rinse when washing glassware The order of amino acids on a polypeptide chain is encoded by: a) DNA b) mRNA c) rRNA d) Regulatory proteins b) mRNA How do DNA molecules vary from one species to another? a) The type of nitrogenous bases they contain b) The number of genes and non-coding regions c) The way nitrogenous bases pair d) The directionality of the strand b) The number of genes and non-coding regions What is agar? a) Solid media on which bacteria grow b) Liquid media in which bacterial grow c) Solid matrix used for DNA gel electrophoresis d) Solid matrix used for vertical gel electrophoresis a) Solid media on which bacteria grow What part of an amino acid differs from one to the other? a) Amino group b) Carboxyl group c) R group d) All of the above c) R group How are the proteins in fractions collected during column chromatography visualized? a) Using a pH meter b) Using agarose gel electrophoresis c) Using polyacrylamide gel electrophoresis (SDS PAGE) d) Using microscopy c) Using polyacrylamide gel electrophoresis (SDS PAGE) Which of the following is an mRNA codon? a) ATA b) UAU c) Tyrosine d) All of the above b) UAU What is the purpose of SDS when used in SDS PAGE electrophoresis? a) Stain the proteins for visualization b) Use as a loading dye c) Denature the proteins and give them a negative charge d) Conduct electricity c) Denature the proteins and give them a negative charge Which department is in charge of testing a biopharmaceutical product to ensure its safety and potency? a) Research and Development (R&D) b) Quality Assurance (QA) c) Manufacturing d) Quality Control (QC) d) Quality Control (QC) Lipids provide which of the following functions for cells? a) Genetic code b) Stored energy c) Transportation d) Structure b) Stored energy Which of the following is a monosaccharide commonly used as an energy source for cells in cell culture media? a) Albumin b) Starch c) Cellulose d) Glucose d) Glucose Where does translation occur in prokaryotes? a) In the endoplasmic reticulum b) At the ribosomes c) Outside the cell d) In the nucleus b) At the ribosomes . In aerobic respiration carbohydrates are ultimately broken down into: a) Acetyl-CoA, H20, ATP b) CO2, H20, ATP c) O2, H20, ATP d) H2O, Acetyl-CoA, ATP e) Heat b) CO2, H20, ATP Most ATP in eukaryotic cells is produced in the: a) Mitochondria b) Nucleus c) Cytoplasm d) Rough endoplasmic reticulum e) Peroxisome a) Mitochondria An aqueous solution and yields a hydrogen ion when ionized acid Defined as "the moles of solute per liter of solution" molarity A solution that resists changes in pH buffer The measurement of the concentration of hydrogen ion in a sample pH Type of test used to measure the amount of total protein present Concentration assays Type of test used to measure the amount of active ingredient in a drug Potency assays Type of test that demonstrates whether or not a specific enzyme or drug is having the desired effect for which it was designed Activity assays A highly specific test to determine protein concentration based on a particular antigen-antibody interaction ELISA A document listing the detailed steps of key processes to help ensure consistent and high-quality output Standard Operating Procedure (SOP) Viscous buffer used to track DNA or proteins during electrophoresis Loading Dye Quantitative measurement of absorbance based on visible light, near-ultraviolet, and near-infrared light sources passing through a solution Spectrophotometry The use of an electric field to separate DNA in agarose Gel electrophoresis The process of making RNA from DNA Transcription The process by which RNA is decoded and polypeptides are formed Translation Autonomously replicating small circles of DNA present in some bacteria Plasmids The deliberate modification of the characteristics of an organism by manipulating its genetic material Genetic engineering