What should you do if the fire alarm rings? - 1. stop experiment
2. if applicable, close windows
3. line up in orderly fashion
4. proceed down steps with last person closing the door
T of F: use a kimwipe to clean the microscope lens - False, use gauze
What happens if an animal cell is placed in a hypertonic solution? - the cell will shrivel
What happens if an animal cell is placed in a hypotonic solution? - the cell will burst
According to the "Selling soap" article when did doctors finally begin to comply washing their hands? -
when they were shown a picture of the bacteria culture from their hands
Peroxidase from turnips is a ______? - enzyme
Hydrogen peroxide is a _______ ? - substrate
What color is guaiacol in its oxidized state? - yellow
What color is DCPIP in its oxidized state? - purple
Compare the leaves when grown in the dark and in the light when their pigment was removed. - Dark:
the leaf is brown and dark; no starch present
light: the leaf is yellow; starch present
Compare the tobacco seeds when grown in the light and dark. - Light: seeds were green
, Dark: seeds were albino
Height and weight/shape in dogs is variable within breeds. This suggests that traits in dogs are _______ -
polymorphic
Three stages of PCR - denaturation (94˚C), annealing (60˚C), extension (72˚C)
iris diaphragm - controls the amount of light passing through the specimen
Parfocality - remains close in focus as it switches from objective to objective, minor adjustments with
the fine focus knob may be necessary
when do you use immersion oil? - 100x lens
binocular dissecting light microscope - also known as dissecting microscope: often used for low
magnification of plate cultures, or for structures that are viewed as live specimens and/or too large to
view under a standard compound microscope; magnifications range from 10x to 25x or 7x to 30x
when defining a solution as iso, hypo, and hypertonic, what process does this relate to? - Osmosis: the
diffusion of water through a semi permeable membrane to an area of higher concentration
Diffusion - Movement of molecules from an area of higher concentration to an area of lower
concentration.
Can osmosis and diffusion occur at the same time? - Yes. Ex: cell solution contained NaCl and a beaker
contained water; NaCl moved into the beaker (diffusion) and water moved into the bag (osmosis)
T or F: 150 mM NaCl and 300mM glucose are osmotic equivalents - True
In the enzyme activity, what is the last solution added to the test tube? Why? - two dropped of
substrate (hydrogen peroxide); this must be added last because the solution starts reacting and must be
covered by Parafilm and placed into the spec for an absorbance reading
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