Southern Blot - CORRECT ANSWER DNA Target
Nucleic acid probe
Purpose: Paternity testing, gene discovery and mapping, mutation analysis
and ID, gene structure
Northern Blot - CORRECT ANSWER RNA Target
Nucleic acid probe
Purpose: Determine transcript structure, processing, and gene expression
,ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
Western Blot - CORRECT ANSWER Protein Target
Protein probe
Purpose: Protein processing, gene expression
Southwestern Blot - CORRECT ANSWER Protein target
DNA probe
Purpose: DNA binding proteins, gene regulation
Eastern blot - CORRECT ANSWER Protein target
Protein probe
Purpose: Modification of Western blot using enzymatic detection (protein
processing, gene expression), detection of specific agriculturally important
proteins
Far-eastern blot - CORRECT ANSWER Lipid target
No probe
Purpose: Transfer separated lipids to membranes for analysis by mass
spectometry
PCR Primers - CORRECT ANSWER Direct DNA synthesis to desired
region
, ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
PCR: dATP, dCTP, dGTP, dTTP - CORRECT ANSWER Building blocks
that extend primers
PCR KCl - CORRECT ANSWER Salt for optimal hybridization of
primers to template
PCR: Tris buffer - CORRECT ANSWER Buffer to maintain optimal pH
for enzyme reaction
PCR: MgCl2 - CORRECT ANSWER Divalent cation, required by enzyme
PCR Polymerase - CORRECT ANSWER Polymerase enzyme that
extends the primers, adds the dNTPs
PCR: copies of template - CORRECT ANSWER Sample DNA that is
being tested
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller TestBankcenter. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.49. You're not tied to anything after your purchase.
