Bio 242 Exam 1 Questions And Answers With Verified
Solutions Latest Update 2024/2025.
Prokaryote - ANSWER A unicellular organism that lacks a nucleus and membrane bound
organelles. Bacteria and Archaea.
Eukaryote - ANSWER organism whose cells contain a nucleus and membrane bound
organelles. Fungi, protozoa, algea, parasatic worms,
Medical Microbiology - ANSWER Deals with microbes that cause diseases in humans and
animals
Public Health Microbiology and Epidemiology - ANSWER monitor and control the spread of
diseases in communities.
immunology - ANSWER Studies the complex web of protective substances and cells produced
in response to infections such diverse areas as vaccination, blood testing, and allergy.
industrial microbiology - ANSWER - safeguards our food and water
- biotechnology
- microbes used to create amino acids, beer, drugs, enzymes, and vitamins
Agricultural Microbiology - ANSWER concerned with the relationships between microbes and
domesticated plants and animals
Enviromental Microbiology - ANSWER •Study the effect of microbes on the earth's diverse
habitats
•Aquatic, soil, and geomicrobiology, and astrobiology
Robert Hooke - ANSWER first to observe (1600) and name cells
Antonie van Leeuwenhoek (1673) - ANSWER Made a crude microscope to examine threads in
fabrics. Made drawings of what he called animacules in rainwater and scraped from his teeth.
Robert Koch - ANSWER Father of microbiology. Linked a specific microorganism with a specific
disease.
Dr. Oliver Wendell Holmes and Dr. Ignaz Semmelweis - ANSWER Showed that women became
infected in the maternity ward after examinations by physicians who had been working in the autopsy
rooms. (Washing hands and desinfection)
Joseph Lister - ANSWER First to utilize hand washing and misting operating rooms with
antiseptic chemicals.
Techniques become the foundation for modern microbial control still in use today.
, Alexander Fleming - ANSWER Discovered penicillin in 1928
Pasteur - ANSWER Invented pasteurization
Showed that human diseases could arise from infection
Robert Koch 2 - ANSWER Established a series of proofs that verified the germ theory of
disease.
Five basic techniques to manipulate, grow, examine, and characterize microorganisms in the
laboratory - ANSWER Inoculation, Incubation, Isolation, Inspection, Identification.
Inoculation - ANSWER *Cultivation of microorganisms
*Any growth that appears in or on the medium after incubation
*Pure culture
*A container of medium that contains only a single known species or type of microorganism
*Used most frequently for laboratory studysample is palce into a perti dish or container of growth
medium.
Inoculation - ANSWER Introdution of microbes into media for culture.
Sterile: free of microbes including endopores
A requirement for any instrument used for sampling and inoculation.
Selective media - ANSWER contains one or more agents that inhibit growth of some microbes
and encourage growth of the desired microbes
Differential media - ANSWER Allow multiple types of microorganisms to grow, but display
visible differences between colonies.
Isolation technique - ANSWER Colony:
•A discrete mound of cells formed on solid nutrient surface
•Consists of just one species and no other if formed from a single cell
mixed culture - ANSWER a container that holds two or more identified, easily differentiated
species of microorganisms
contaminated culture - ANSWER A culture that was once pure or mixed that now contains
contaminants, or unwanted microbes of uncertain identity
Properties of a light microscopy - ANSWER magnification, resolution, contrast
Incubation - ANSWER proper growth conditions, temperature and gas requirements.
Isolation - ANSWER Cultures are isolated, reinoculated and reincubated to obtain a single
species sample.
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