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Lab techniques part 1 readings

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yeast properties - answerrapid growth, dispersed cells, ease of replica plating, mutant isolation, and DNA transformation isolating recessive strains - answermutations isolated in haploid strains with complementation tests carried out in diploid strains yeast transformation - answerutilizes s...

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  • August 5, 2024
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Lab techniques part 1 readings
yeast properties - answerrapid growth, dispersed cells, ease of replica plating, mutant isolation,
and DNA transformation

isolating recessive strains - answermutations isolated in haploid strains with complementation
tests carried out in diploid strains

yeast transformation - answerutilizes synthetic oligonucleotides

YACs - answerused to clone large fragments of DNA

Saccharomyces - answeryeast with no true wild-type strain

S288C - answercontains defective HAP1 gene and incompatible for mitochondrial systems and
does not form psuedohyae

psuedohyae - answersynchronously dividing elongated yeast cells expressed as branched
chains growing outward from the center of the colony and invasive growth under surface of agar
medium
usually observed w S. cerevisiae on agar medium limiting Nitrogen

D273-10B - answerused as typical yeast for mitochondrial studies

budding - answerdaughter initiated as an outgrowth from mother cell, then nuclear division, cell
wall formation, and lastly cell separation

diploid cell size - answer5 x 6 um ellipsoid

haploid cell sizr - answer4um diameter

mother cells - answerform no more than 20-30 daughters
age determined by scars on cell wall

haploid budding - answerhappens adjacent to previous cell

diploid budding - answerbuds appear at opposite poles

haploid growth optimal temp - answer30 degrees celsius

strain maximum density in YPD medium - answer2 x 10^8

sporulation - answerdiploid cells undergo meiosis --> 4 progeny haploid that become
encapsulated as spores

ascus - answersac-like structure surrounding spores

S. cerevisiae chromosomes - answerhaploid set of 16 chromosomes

yeast tRNA genes - answer262 tRNA genes with 80 introns

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