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ASCP MB Exam Extra Practice Questions With Correct Answers 2024
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ASCP MB Exam Extra Practice Questions With Correct Answers 2024
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ASCP MB Exam Extra Practice QuestionsWith Correct Answers 2024
The fKlenow ffragment fof fE. fcoli fDNA fpolymerase fI fis fused: f- fcorrect fanswer.to flabel fDNA
fprobes fby f random fprimer f labeling.
What fis fa fsolution fhybridization fmethod ffor fthe fdetection fof fnucleic facid:protein
f interactions? f - fcorrect fanswer.gel fmobility f shift fassay
_____ finvolves fmixing fa flabeled foligonucleotide fwith fa fprotein fextract fto fsee fif fa fspecific
f interaction foccurs, f visible f with fan f apparent f increase f in fmolecular f size fon fa f gel fdue fto fthe
fprotein:nucleic facid finteraction. f - fcorrect f answer.Gel fmobility f shift fassay
Which fprobe fis fmost foften fused fto fdetect ftrisomy f21 fby finterphase fFISH? f- fcorrect
fanswer.alpha f satellite fDNA f probe
_____ fdetect fspecific fchromosomes fand fnumbers fof fthe fspecific fchromosomes. f- fcorrect
fanswer.Alpha f satellite fDNA f probe
Which freagent fgenerates fa fsignal fduring fthe fannealing fstage fof fa fquantitative freal ftime
fPCR f reaction? f - f correct f answer.molecular fbeacon
Methylation fof fcytosine fresidues fis finvolved fin fthe falteration fof fgene fexpression fin fa
fnumber fof fcancers. fWhich f sequencing ftechnique f is fbest f suited fto fdetermine f if
fmethylation fof f cytosine f residues fhas ftaken fplace f in f the f promoter f region fof f a f given f gene?
f - f correct fanswer.bisulfite f sequencing
_____ fis fa fmodified fchain fterminator fmethod fspecifically fdesigned fto fdetect fmethylated
fnucleotides. f - f correct fanswer.Bisulfite f sequencing
Which fmethod fis fbest fsuited fto fscreen ffor fmutations fin fa fgene fhaving fmultiple fpossible
fmutations? f - fcorrect fanswer.single f strand fconformational fpolymorphism f (SSCP)
_____ fis fa fscreening ftest fin fwhich fthe fexact fnature fof fa fmutation fdoes fnot fneed fto fbe
f known f in forder fto f detect f the fpresence fof fa fmutation. f - f correct fanswer.Single f strand
f conformational fpolymorphism f (SSCP)
,What fis fthe fbest fmethod fto frule fout fthe fpossibility fof fgenomic fDNA fgiving fa ffalse-positive
f result f in fan f RT-PCR f reaction? f - fcorrect f answer.use fof f upstream fand fdownstream
fprimers fthat f span fan fexon-intron-exon f region fof fthe f target
*amplicaons fgenerated ffrom fgenomic fDNA fwould fbe flarger fthan fthe fspecific famplicon
f generated ffrom fa fmature, f spliced fmRNA
A fcommon fuse ffor fpulsed-field fgel felectrophoresis fis: f- fcorrect fanswer.epidemiological
ftyping fof fbacterial fstrains.
_____ fafter fdigestion fof fbacterial fchromosomes fwith frestriction fenzymes fis fused fto
fdetermine fsimilarity f between f bacterial fisolates. f - fcorrect fanswer.PFGE
A ftranslocation fin fwhich fchromosome fpair fcreates fa fBCR/ABL1 ffusion fgene fproduct
fassociated f with f CML?
a. f11;18
b. f14;18
c. f9;22
d. f9;14 f- fcorrect fanswer.t(9;22)
The fpresence fof fa fdistinct fband falong fwith fthe fsmear findicates fat fleast f1% fof fthe fpatient's
fB fcells fhave fa f_____ fof f the fIg fheavy f chain. f - fcorrect fanswer.monoclonal f rearrangement
A fmolecular-based ftest fcommonly fused fin ftesting fdonors fand frecipients fprior fto forgan
ftransplantation f is: f - fcorrect fanswer.sequence-specific fPCR.
_____ fis fcommonly fperformed fto fdetermine fpolymorphisms fin fclass fI fand fclass fII fHLA-
DRB fand fHLA-DRQ fgenes. f- fcorrect fanswer.Sequence-specific fPCR
DNA fsequencing fof fhypervariable fregions fI fand fII fis fused fto: f- fcorrect fanswer.detect
fpolymorphisms fin fhuman fmitochondrial f DNA.
HV1 fand fHV2 fare fsequenced fto fdetermine f_____. f- fcorrect fanswer.mitochondrial
f inheritance.
Repeated fdiscrepancies fbetween fpatient fidentifiers fwill fresult fin f_____ f- fcorrect
fanswer.the f error fbeing fdocumented fto f the f laboratory f supervisor fand fthe frisk fmanager
f will f conduct f a f root fcause fanalysis.
An findex fof fprecision fis fstatistically fknown fas fthe: f- fcorrect fanswer.coefficient fof fvariation.
The f_____ fis fthe fmeasure fof frelative frandom ferror fexpressed fas fa fpercentage. f- fcorrect
fanswer.coefficient fof f variation
The fterm fused fto fdescribe freproducibility fis: f- fcorrect fanswer.precision.
, _____ fis fthe fdegree fto fwhich fresults fof fmultiple fanalyses fof fthe fsame fspecimen fagree. f-
f correct fanswer.Precision
The fprocess fby fwhich fan fagency for forganization fuses fpredetermined fstandards fto
fevaluate fand f recognize fa fprogram fof f study f in fan f institution f is fcalled: f - f correct
fanswer.accreditation.
Using fa fcommon flabeling fsystem ffor fhazardous fmaterial fidentification fsuch fas fHMIS for
f NFPA f704, f which fhazard f is f represented fby:
the ftop fred fquadrant?
the fright fyellow fquadrant?
the flower fwhite fquadrant?
the fleft fblue fquadrant? f- fcorrect fanswer.flammability
reactivity
special freactivity
health
If fthe fHMIS for fNFPA f704 fhazardous fmaterial fidentification fsystem fhas fa fnumber f4 fin fthe
f left fblue f quadrant, fit f represents fa: f - f correct fanswer.high f health fhazard
The fratings ffor fthe fHMIS for fNFPA f704 fhazardous fmaterial fidentification fsysthem fare f0-4
f with f0 fbeing f _____ fand f4 fbeing f_____. f - f correct fanswer.no fhazard; fmost f severe f hazard
Which fchemical fis fa fpotential fcarcinogen? f- fcorrect fanswer.formaldehyde
When fhazardous fchemicals fare ftransferred ffrom fthe foriginal fappropriately flabeled
f container(s) f to fa fsecondary f container ffor fimmediate fuse f by f the f person fperforming f the
ftransfer, f it: f - f correct fanswer.does f not frequire f labeling.
A ftechnologist fspilled f10 fgallons fof fformaldehyde fon fthe ffloor. fAfter fdetermining fthe
f chemical fposes fa fsignificant fhealth fhazard, fthe ffirst f action f step f would fbe fto: f - f correct
fanswer.evacuate f the f area.
One fof fthe felements fof fa fwritten flaboratory fchemical fhygiene fplan fis fto: f- fcorrect
fanswer.designate fa f laboratory f chemical fhygiene f officer.
When fworking fwith fsharp fequipment fand fobjects, fuse fa: f- fcorrect fanswer.mechanical
fdevice.
Incident freports ffor foccupational finjury for fillness fshould: f- fcorrect fanswer.be ffiled ffor fall
f incidents f including fnear f miss fincidents.
According fto fOSHA, fwhat ftype fof fwarning fsign fshould fbe fposted fin fan farea fwhere fan
f immediate f hazard f exists f and f where fspecial f precautions fare f necessary? f - f correct
fanswer.red, fblack fand fwhite f"Danger" f sign
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