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MCB4304 Exam 1 Final Questions & Answers 2024/2025
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MCB4304 Exam 1 Final Questions & Answers 2024/2025
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MCB4304 Exam 1 Final Questions &Answers 2024/2025
What two types of probes are widely used - ANSWERSPolynucleotides, also called oligonucleotides
and Antibodies
What are two popular plasmids used as vectors - ANSWERSpBR322
pUC
what is pBR322 - ANSWERS2 antibiotic resistant genes
Variety of unique restriction sites
Most sites interrupt antibiotic resistance, making screening straightforward
What is pUC - ANSWERSAmpicillin resistance gene
MCS within the 5 fragment of b galactosidase gene
What do probes do? - ANSWERSProbes are used to identify a desired clone from among the thousands
of irrelevant ones
What is a cDNA library? - ANSWERSA cDNA library is a set of cones representing as many as possible
mRNAs in a given cell type at a given time
What is (RACE) and what does it do - ANSWERSRapid Amplification of cDNA ends
If generated, cDNA is not full-length missing pieces can be filled I using rapid amplification of cDNA ends
(RACE)
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