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CHEM 317 BIO CHEMISTRY I EXAM 3 Questions and Answers to Score 100% PORTAGE LEARNING
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CHEM 317 BIO CHEMISTRY I EXAM 3 Questions and Answers to Score 100% PORTAGE LEARNING
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lOMoAR cPSD| 42147428CHEM 317 BIO CHEMISTRY I
EXAM 3 Questions and Answers to
Score 100% PORTAGE
LEARNING
, lOMoAR cPSD| 42147428
CHEM317-001-202120 Biochemistry I
Question 1
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Question text
You are studying a new restriction enzyme that recognizes and cuts at the sequence 5’-
GCGCNNNNNGCGC-3’. How big on average do you expect the restriction fragments to
be when you digest the genomic DNA? Assume that the genomic DNA sequence is
random. N means could be any nucleotides: A, T, G, or C.
Select one:
a. ~4 kb
b. ~1 kb
c. ~250 bp
d. ~16 kb
e. ~64 kb
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Your answer is correct.
The correct answer is: ~64 kb
Question 2
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To express human insulin in E.coli cell requires DNA recombination technique. The
following figure shows DNA fragments: A, B, and C. They were cloned into a plasmid.
The figure shows restriction enzyme sites: EcoR I, Hind III and Sac I of an expression
vector.
The correct expression vector will be . If this expression vector is
cleaved by EcoR I, and Hind III, how many DNA fragments will be seen in gel
electrophoresis?
Select one:
a. A: human promoter; B: human insulin cDNA; C: human terminator; 2
b. A: bacterial promoter; B: human insulin cDNA; C: bacterial terminator; 2
c. A: either bacterial or human promoter; B: human insulin cDNA; C: either bacterial or human
terminator; either 2 or 3
d. A: human promoter; B: human insulin cDNA; C: human terminator; 3
e. A: bacterial promoter; B: human insulin cDNA; C: bacterial terminator; 3
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Your answer is correct.
The correct answer is: A: bacterial promoter; B: human insulin cDNA; C: bacterial terminator; 3
Question 3
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