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Biol 227 Lab Exam; Questions and Answers 100% Correct $12.99   Add to cart

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Biol 227 Lab Exam; Questions and Answers 100% Correct

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  • Bio 227

Biol 227 Lab Exam; Questions and Answers 100% Correct body of microscope Answer-arm and base light source Answer-Projects light upwards through the diaphragm, the specimen, and the lenses. Has on/off switch and intensity knob eyepiece Answer-Contains the ocular lens ocular lens Answer-magni...

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  • August 8, 2024
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  • Bio 227
  • Bio 227
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Biol 227 Lab Exam; Questions and Answers
100% Correct
body of microscope Answer-arm and base




light source Answer-Projects light upwards through the diaphragm, the specimen, and
the lenses. Has on/off switch and intensity knob




eyepiece Answer-Contains the ocular lens




ocular lens Answer-magnifies the image 10x




total magnification Answer-objective lens x ocular lens




field of view Answer-The area visible through the microscope eyepiece




objective lens Answer-the part of a compound light microscope that is located directly
above the specimen and that magnifies the image of the specimen (4x, 10x, 40x, 100x)




working distance Answer-distance between objective lens and specimen




stage of microscope Answer-Holds the slide or object you are looking at. (move with x
and y knobs)

, Biol 227 Lab Exam; Questions and Answers
100% Correct
focus adjustment knobs Answer-course adjustment knob for larger focusing and fine
adjustment knob for precision focusing




condenser Answer-focuses light through the specimen




condenser focus knob Answer-raises and lowers the condenser relative to the stage
(vertical height adjustment)




diaphragm Answer-Regulates the amount of light on the specimen




aperture iris diaphragm lever (contrast lever) Answer-Adjust intensity of image in precise
increments; left increases intensity, right decreases intensity




field iris diaphragm Answer-controls the amount of light entering the condenser




interpupillary distance Answer-distance between eyepieces




diopter adjustment Answer-Useful as a means to change focus on one eyepiece so as to
correct for any difference in vision between your two eyes.




Using a microscope Answer-Clean lenses, eyepiece, and objectives with lense paper

, Biol 227 Lab Exam; Questions and Answers
100% Correct
turn on light to midlevel

Raise condenser to highest position

Place slide on stage and center

Using 100x objective, raise stage until slide almost touches

Find specimen with 4x

Rotate 10x and bring into focus with fine adjustment

Adjust interpupillary distant and diopter adjustments

Kohler illumination




Purpose of Kohler illumination Answer-Allows for high contrast and resolution with
reduced heat on the specimen




Kohler illumination Answer-Contrast level/aperture iris to middle

close field iris diaphragm, center the circle

lower condenser until polygon is clear

open field iris to edge of FOV




bright field Answer-dark specimen on bright background




dark field Answer-bright specimen on dark background (good for transparent specimen)

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