Exam (elaborations)
LSU CHEM 317 BIOCHEMISTRY I FINAL EXAM Questions and Answers Louisiana University
- Course
- Institution
LSU CHEM 317 BIOCHEMISTRY I FINAL EXAM Questions and Answers Louisiana University
[Show more]
Content preview
LSU CHEM 317 BIOCHEMISTRY IFINAL EXAM Questions and Answers
Louisiana University
CHEM317-001-202120 Biochemistry I
Started on Monday, 23 November 2020, 8:00 AM
State Finished
Completed on Monday, 23 November 2020, 10:30 AM
Time taken 2 hours 29 mins
Marks 43.00/67.00
Grade 129.00 out of 201.00 (64%)
Question 1
Correct
Mark 1.00 out of 1.00
Flag question
Question text
The antibiotic fluconazole is used against pathogenic fungi. After long-term treatment,
mutations of the organisms can arise. One yeast strain was mutated. The kinetics of the
enzyme that is the target of fluconazole, lanosterol 14-demethylase (14-DM), were
determined in the wild type and the mutant yeast, and curves shown in Figure 1a and
1b were obtained. In order to determine the mutations in 14-DM, the amino acid
sequences of 14-DM from the wild type and the mutant strain were determined. Figure
2 shows a comparison of the part of the sequences where differences were found.
,Which of the following is the effect of fluconazole on the lanosterol 14-DM? Which of the
following amino acid changes shown in Figure 2 is most likely to alter sensitivity to
fluconazole?
Select one:
a. Noncompetitive; Lys → Arg
b. Competitive inhibition; Lys → Arg
c. Noncompetitive; His → Thr
,d. Competitive inhibition; His → Thr
e. Either competitive inhibition or noncompetitive inhibition; Ether Lys → Arg or His → Thr
Feedback
Your answer is correct.
The correct answer is: Noncompetitive; His → Thr
Question 2
Not answered
Marked out of 1.00
Flag question
Question text
Protein concentration can be quantitated by visible spectrometry. In one experiment,
the standard curve below was produced. A 10 μL aliquot of protein isolate was diluted
to a final volume of 100 μL with buffer. The absorbance of this dilution was 0.61. What
is the protein concentration of the original isolate?
Select one:
a. 0.151 mg·mL-1
b. 1.51 mg·mL-1
c. 0.0151 mg·mL-1
d. 15.1 mg·mL-1
e. 151 mg·mL-1
Feedback
Your answer is incorrect.
The correct answer is: 0.151 mg·mL-1
Question 3
, Correct
Mark 1.00 out of 1.00
Flag question
Question text
Millions of children that depend primarily on rice as a food staple become blind each
year due to vitamin A deficiency. Humans cannot synthesize vitamin A and must have a
supply of vitamin A or vitamin A precursor like beta-carotene in their diet. You want to
create a strain of yeast that has the complete beta-carotene pathway so you can easily
study the biochemistry of beta-carotene and vitamin A synthesis. You have a yeast
strain that has 6 of the required 7 enzymes for beta-carotene synthesis. You need to
provide these yeast cells with the missing enzyme psy2 from daffodils. You are also
given some DNA sequence from the psy2 locus, cloning vector 2 and restriction
enzymes as shown below.
To complete production of the yeast strain capable of synthesizing beta-carotene, you
decide to use PCR to amplify the psy2 coding sequence based on the flanking sequence
shown below
Select one:
a. Sense primer: 5’-AGGCCG-3’; Anti-sense primer: 5’-GCCGGA-3’
b. Sense primer: 5’-AGGCCG-3’; Anti-sense primer: 5’-ACCGGG-3’
c. Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-ACCGGG-3’
d. Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-GCCGGA-3’
e. Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-TCCGGC-3’
Feedback
Your answer is correct.
The correct answer is: Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-GCCGGA-3’
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller AcademicAllure. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $15.89. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75619 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now