Cell Biology Lab Final Exam
absorption spectrum - ANS-A plot of various wavelength versus their absorption is an
all of the above - ANS-Absorption spectrum can be used to identify
x rays, ultraviolet, visible, infrared, microwave - ANS-what is the order of electromagnetic
radiation (from short wavelength to longer)
standard curve - ANS-A plot of several protein concentration verses their corresponding
absorptions is called
biuret - ANS-In which of the following assays for quantification of protein concentration, the
colored product is as a result of coordination of protein peptide bond with alkaline copper sulfate
along with running actual test for unknown detection - ANS-a calibration curve (standard curve)
for any biochemical test must be prepared
a purified preparation of protein to be tested - ANS-the best protein to be used as a standard
protein to generate a standard curve is
Destructive to the protein - ANS-In comparison with other assays for protein measurement,
direct spectrophotometric methods, IS NOT
Bradford - ANS-A protein-dye complex is formed when using one of the following method
aromatic rings of nucleotides - ANS-The intense ultraviolet absorption by nucleic acid molecules
is due to presence of
aromatic rings of amino acids - ANS-The intense ultraviolet absorption by protein molecule is
due to presence of
200nm - ANS-nucleic acis display intense absorption at
Direct spectrophotometric )(A280) - ANS-if you have a relatively high concentration of nucleic
acid in your protein extract, which of the following methods is less appropriate for determination
of protein concentration
, Electronic (inner shell) - ANS-X ray crystallography
Vibrational/ rotational - ANS-Raman spectroscopy
electronic (otter shell) - ANS-nuclear magnetic resonance
Nuclear - ANS-Fluorescence spectroscopy
X ray - ANS-Which of the following radiations has the highest energy
WHite - ANS-If a sample reflect all of the visible wavelengths, it appears
Produce a three dimensional picture of density of electrons within the crystal - ANS-When a
beam of X ray strikes a crystal
all of the above - ANS-Cell culture technique in general refers to
first human cell culture developed from her cervical cancer tissue - ANS-Henrietta Lacks
A & C - ANS-Most primary cell culture have limited lifespan unless
cell growth arrest - ANS-The growth factors (FBS, Fetal Bovine Serum) used to supplement cell
culture media often derived from animal blood which can potentially be a source of
cell splitting - ANS-In cell culture technique, transferring small number of cells into a new vessel
called
all of the above - ANS-Nutrient depletion in growth media of cell culture causes
Cell grow as a suspension - ANS-Adherent cell culture are cells
B & C - ANS-Tissue engineering and stem cell industry are based on
80-90% - ANS-At what confluency the cells need to be splitted
1. Calculate concentration of unknown using direct spectrophotometer
2. Identify unknown compound using absorbance spectrum
3. Measurement of color intensity to calculate concentration - ANS-NAME THREE
APPLICATION OF SPECTROPHOtoMETRIC TECHNIQUE
polyacrylamide with high concentration - ANS-What type of support media should be used to
characteize a 5-100 base pair fragment DNA
all of the above - ANS-Proteomic, is discipline that
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