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Cell Biology Lab 2 Detailed Questions And Expert Answers
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Cell Biology Lab 2 Detailed Questions And Expert Answers
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Cell Biology Lab 2 Detailed Questions And ExpertAnswers
Paper chromatography is a technique to separate molecules in a mixture based on
what? - ANS their solubility in an organic solvent and water
What basis does chromatography separate pigments by? - ANS it separates them
based on their ability to be distributed between mobile and stationary phases. In
this specific lab, the solubility of pigments in water (stationary) vs in an organic
solvent (mobile)
Is the pigment higher up on the chromatography paper mobile or stationary? Polar
or nonpolar? - ANS higher pigment is nonpolar, soluble in organic solvent, and
mobile. The lower pigment is polar, soluble in water, and stationary
What is the equation for Rf and what does it mean? - ANS RF= the distance
traveled by the solute/distance traveled by the solvent. The closer this value is to
one, the more soluble and mobile it is
What does a spectrophotometer do? - ANS it measures the amount of light
absorbed or transmitted by a sample by passing monochromatic light through it
What is the beer lambert law? - ANS A=acl where A is absorbance
a is constant for solute (extinction coefficient)
c is the concentration of solute
, l is the length of the light path
What two specimen do we use in our chromatography lab? - ANS spinach and
swiss chard
What does chromatography do? - ANS separate molecules in a mixture based on
their ability to be distributed between a mobile phase and a stationary phase
What does the distribution between mobile and stationary phases depend on? -
ANS the chemical nature of the molecules and the ability of the molecules
andphase to interact
What is the basis for the paper chromatography used in this experirment? - ANS
the differential solubility of the pigments in water and an organic solvent
What represents the stationary phase? - ANS solubility in water
What represents the mobile phase? - ANS the solubility in the organic solvent
What will happen to the pigments that are more soluble in the organic solvent? -
ANS They will travel with the solent as it is wicked up the filter paper
What will happen to the pigments that are more soluble in water? - ANS They will
not move as rapidly and will be closer to the origin of application
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