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Cell Biology: Lab 2 Questions And Expert Answers
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Cell Biology: Lab 2 Questions And Expert Answers
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Cell Biology: Lab 2 Questions And Expert AnswersWhat is totipotency? - ANS The property that each cell of a multicellular organism
has all of the genes required to form an entire new organism identical to the
original
Outline how you will prepare the carrot explants - ANS 1. Cut the taproot into
pieces 5 to 7 cm long. Immerse the pieces for 30 seconds and 70% alcohol, then
transferred to a beaker of 10% Clorox. Stir for 15 minutes. Rinse three times with
sterile distilled water, 5 minutes/rinse.
2. Remove the cork borer from alcohol and flame it. Insert it into the carrot piece,
pull the cork bore out of the carrot and place the remaining carrot on a sterile
surface. Use a glass rod dipped in ethanol to push the core from the cork borer
into a sterile petri dish.
3. Flame forceps and scalpel to remove alcohol. With the scalpel, cut off about 1
cm from the ends of the core. Cut meaning carrot core into 2 to 4 mm cross
sections. Try for 5 or 6 pieces
4. Take a dish of sterilized callous initiation medium in carefully lift the lid away
from you. Do not remove it! Using sterile forceps place the cross-sections of
character on the medium. Spread them out from one another. Close the dish and
seal it with parafilm. Mark the dish with the date, your name, and lab section.
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