Exam (elaborations)
ASCP CG CERTIFICATION PRACTICE QUESTIONS AND ANSWERS WITH SOLUTIONS 2024
- Course
- Institution
ASCP CG CERTIFICATION PRACTICE QUESTIONS AND ANSWERS WITH SOLUTIONS 2024
[Show more]
Content preview
ASCP CG CERTIFICATION PRACTICEQUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
What is the best agent to use against mycoplasma? - ANSWER BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: - ANSWER
comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ANSWER Proteolytic enzymes - break down tissues into single-
cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation
with: - ANSWER Colcemid - mitotic spindle poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ANSWER Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? - ANSWER T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is the MOST
important to document? - ANSWER Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - ANSWER Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? - ANSWER Decidua
(maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the
mets, what is the most likely cause? - ANSWER innappropriate slide drying time
,What will help prevent microbial contamination and cross contamination of cultures? - ANSWER Asceptic
technique
When there are not enough mets to complete the study of a patient/case, what needs to be done next? -
ANSWER prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what
could help fix the issue? - ANSWER Increading the drying time by using warm slides
What is true of an inverted microscope? - ANSWER the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - ANSWER DiGenorge syndrome --
microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST important data to
accompany the specimen? - ANSWER Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? - ANSWER
Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: - ANSWER Dividing
leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to monitor to
assess the source of the problem? - ANSWER Temperature,
, CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase contrast scope is:
- ANSWER Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down syndrome
translocation carrier? - ANSWER 45
What is the best tissue to use for both a good success rate and abnormality detection in spontaneous
abortion miscarriages? - ANSWER Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? - ANSWER The door
does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up amniotic fluid
samples, the specimen could become contaminated with: - ANSWER Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain used on
the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid contamination
What about the metaphases on a slide are affected by slide making? - ANSWER Cell density - thru
dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi syndrome? - ANSWER High
resolution banding and FISH for the Prader-Willi locus on 15q11.2
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Performance. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $14.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
73091 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now