Microbiology Lab Final UTA Questions
And Answers With Verified Solutions
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5 Implicit I's of Microbiology - ANSWER✔✔ Inoculation
Incubation
Isolation
Inspection
Identification
Inoculation - ANSWER✔✔ Put bacteria on/in a medium
Incubation - ANSWER✔✔ Let it grow at an optimum temperature
Isolation - ANSWER✔✔ Separate out individual colonies
Inspection - ANSWER✔✔ Look at characteristics
Identification - ANSWER✔✔ Identify microbe based on observations
Form - ANSWER✔✔ Shape of the whole colony; First thing we see when we
examine a bacterium under the microscope
Margin - ANSWER✔✔ Edge of colony
,Elevation - ANSWER✔✔ Side view
Morphology - ANSWER✔✔ Form, Margins, Elevation, and Pigment Production
Friable - ANSWER✔✔ Crusty growth
Agar Slants - ANSWER✔✔ Used mainly in media cultivation and maintenance of
stock cultures.
-Dry, flat, spreading edge, friable, translucent, mucoid/butyrous, and filiform
growth
Filiform - ANSWER✔✔ Dense opaque growth with a smooth edge
Bacteria are Defined by? - ANSWER✔✔ Their cellular structure and small size
Pellicle - ANSWER✔✔ Bacteria float on top of medium and produce a type of
surface membrane
Sediment - ANSWER✔✔ Bacteria sink to the bottom
Uniform Fine Turbidity (UFT) - ANSWER✔✔ cloudy growth
Flocculent - ANSWER✔✔ Clumping growth
,Coccus/Cocci - ANSWER✔✔ Spherical shape, greek for berry
Can occur in chains (streptococci), clusters (staphylococcus), or tetrads (groups of
four)
Bacillus/Bacilli - ANSWER✔✔ Rod shaped, latin for little staff
Rounded, flat tapered ends (fusiform) and can either be motile or non-motile
Spirillum/Spirilli - ANSWER✔✔ Rigid curved or spiral shaped bacteria, latin for
little coil
Vibrio - ANSWER✔✔ Gently curved rods
Spriochetes - ANSWER✔✔ Slender, flexible spirals, greek for coil of long hair
Filamentous Bacteria - ANSWER✔✔ Form long multi-nucleated filamentous
hyphae that may branch to produce mycelium
Pleomorphic - ANSWER✔✔ Misc. category
-Many bacterium are variable in shape and cannot be characterized by a single
form. Most of these bacteria assume general rod shapes (different from bacilli)
and can assume squares, star shapes, and cocci-bacilli
Bacterial Smear - ANSWER✔✔ Emulsion of bacteria, spreading bacteria on glass
slide
, Smear prepared from liquid (broth) or from an agar plate (requires drops of
water). Add DI water when smearing from a plate to the slide but not when
smearing broth!
Should not be too thick or thin
Always vortex liquid media beforehand
Why do we stain bacteria? - ANSWER✔✔ Bacteria have same refractive index as
water. These allow cells and internal structures to become more visible
Heat Fixation - ANSWER✔✔ -Heat fix slide only after preparing a bacterial smear
-Pass slide through Bunsen burner flame using a microscopic slide holder
Pros of Heat Fixation - ANSWER✔✔ Kills the bacteria
Fixes bacteria to the slide, ensures against specimen decay over time
Helps with biochemical reactions
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