Microbiology Lab Midterm (Lab Notebook)
Questions and Answers (100% Pass)
What is located in the headpiece that bends light rays toward the ocular lenses? -
✔️✔️prism
Name the only type of paper and three fluids that may be used to clean objective and
ocular lenses? - ✔️✔️-lens paper
-acetone
-xylene
-ethyl alcohol
What is the most important aspect in caring for the microscope? - ✔️✔️keeping it clean
and using it properly
Which way does the stage move when you rotate the coarse adjustment knob towards
you? - ✔️✔️down
Make a statement concerning the distance the stage moves up or down when using the
coarse adjustment compound to the fine adjustment. - ✔️✔️Fine adjustment: sharpens
image
Coarse adjustment: moves the stage and provides the most focus by changing distance
between lens and specimen
Your microscope is equipped with a series of 3 lenses and is therefore called a _______
microscope. - ✔️✔️compound
An image is produced when light is absorbed by a dark opaque object. The background
has a bright white background. Your microscope is therefore also called a ________
microscope. - ✔️✔️bright field
The lenses nearest your eyes are the eyepieces or ______ lenses. - ✔️✔️ocular
What is the magnifying power of there lenses? - ✔️✔️10x
What is the collective name for the four lenses located nearest to the object being
viewed? - ✔️✔️objective lenses
What is the name and magnification of the red banded lens? - ✔️✔️4x
scanning lens
What is the name and magnification of the yellow banded lens? - ✔️✔️10x
low power lens
What is the name and magnification of the blue banded lens? - ✔️✔️40x
high power lens
What is the name and magnification of the white banded lens? - ✔️✔️100X
oil immersion lens
Beginning with the light source, what 3 lenses of the compound light microscope does
light pass through before it reaches your eye? - ✔️✔️1. condenser lens
2. objective lens
3. eyepiece/ocular lens
What is the mathematical definition of resolution power? - ✔️✔️the shortest distance at
which two objects can be visually separated
The shortest visible wavelength of light is _____ nanometers (nm) - ✔️✔️420nm
Convert 420nm into micrometers - ✔️✔️.42micrometers
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