Case
Case BSC 120L
- Course
- BSC 120L
- Institution
- Marshall University
An academic paper about an experiment on the the blood glucose levels in people who are diabetic and non diabetic.
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The comparison of Blood Glucose Levels in Patients who are Diabetic vs Non-DiabeticKimberly Bail
BSC-120L-206
Abstract
Diabetes is a health condition that occurs in a person when their body is not able to
regulate the amount of glucose in their blood. There are two types of diabetes: Type 1 where the
body produces either no insulin or very little insulin and Type 2 where the body is not able to
regulate the insulin. The poor insulin level then allows the glucose in the bloodstream to stay
longer in the body which affects and can damage the blood vessels, the eyes, and multiple other
systems in the body. This study is meant to test the hypothesis that the absorbency rate in people
who are nondiabetic will be lower than in the diabetic people who will have a higher glucose
count. Further research and studies can go in depth with the effects of administering insulin as a
kind of treatment for diabetes. They could also explore the impact of meal composition and
exercise on glucose absorption and the time required for glucose levels to stabilize.
Introduction
In the United States a vast amount of people has diabetes specifically Type 2 due to it
having the possibility to develop and not just being born with it. Typically, the body is able to
regulate glucose levels by maintaining homeostasis and producing insulin to remove any excess
amount of glucose. However, diabetes is a disease in which a person’s body is not able to
regulate the amount of glucose in the bloodstream properly. The glucose then runs through the
bloodstream unchecked and unregulated which can lead to dangerously elevated levels and affect
the blood vessels, the eyes, and multiple other systems in the body and cause damage that could
be irreversible (Centers for Disease Control and Prevention, 2022).
, In this study, the hypothesis that is being tested is that the absorbency rates in non-
diabetic people will be lower than diabetic people. This is thought to be due to the diabetic
samples having a higher amount of glucose that need to be absorbed and regulated. The glucose
levels of diabetic and non-diabetic samples will be compared using spectrophotometry.
Methods And Materials
This study was conducted by creating samples of simulated blood that was considered
either from a diabetic or non-diabetic person. Four different samples were created, these samples
were to show a time frame of about 2 hours from 0 minutes which would be just before eating a
meal, 30 minutes, 60 minutes, 90 minutes, and 120 minutes after eating. The samples would then
be run under a spectrophotometer to measure the blood glucose of the samples which in these
simulations is the amount of light absorbed in each solution. The spectrophotometer is set to a
wavelength of 504nm. Groups were formed and separated based on the fact they either received
a sample of the simulated diabetic blood or non-diabetic blood. The absorbance value of each
sample is then recorded in a table. Then those results are gathered to create an average to
produce the necessary data for this experiment.
Results
1.6
1.4
1.2
Absorbance
1
0.8
0.6
0.4
0.2
0
0 20 40 60 80 100 120 140
Time (minutes)
Non-diabetic Diabetic
Fig 1. The difference of absorbency of
Glucose in the simulated blood over Time
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