ASCP CG Certification Practice 1 Latest Update
2024-2025 170 Questions and 100% Verified
Correct Answers Actual Exam Guaranteed A+
"Starring-in" is a method of - CORRECT ANSWER: Immobilizing the tissue in the culture
vessel
24 hours after culture initiation, a single dish from an amniotic fluid turns cloudy. Which
cultures should be disposed? - CORRECT ANSWER: The single cloudy dish only
46,XY,der(9)(t(9;10)(q32;q12). What term best describes this written karyotype? -
CORRECT ANSWER: Unbalanced
("der" means "a derivative chromosome"; "t" translocation. This indi. has only one copy
of chromosome 9q from band 9q33 to 9qter), therefore is unbalanced
50% of cells from an amniotic flask have 92,XXYY and the other 50% have 46,XY. This
probably indicated: - CORRECT ANSWER: Pseudomosaicism
A blood sample can be used to study the cytogenetics of a malignancy only if: -
CORRECT ANSWER: Dividing leukemic cells are present in circulation
A bone marrow sample collected in transport medium is leaking into the sealed plastic
bag in which it was transported. The technologist should_______. - CORRECT
ANSWER: Clean up the tube while wearing gloves
A change of 1 pH unit in culture medium is caused by a ________ change in CO2
concentration. - CORRECT ANSWER: 10-fold
A Cri-du-chat patient has a chromosome abnormality of: - CORRECT ANSWER:
r(5)(p15.1q35)
,(this ring chromosome is missing the tip end of 5p and the tip end of 5q. Deletion of
5p15 is cri-du-chat syndrome)
A fetus with an increased risk for trisomy 18 would have a serum analytes that is: -
CORRECT ANSWER: low AFP, low hCG, and low uE3
AFP = Alpha feto protein
hCG = human chorionic gonadotropin
uE3 = Unconjugated estriol
A laboratory' quality assurance program includes _______________. - CORRECT
ANSWER: Proficiency testing
A MLL FISH gave the following signal pattern 1F 1R 1G. What is the ISCN of this cell? -
CORRECT ANSWER: nuc ish (MLLx2)(5?MLL sep 3?MLLx1)
A new hypotonic solution causes overspreading of chromosomes. What technique can
be used to prevent the overspreading? - CORRECT ANSWER: Reduce the drying time
A regulatory inspecter requests a copy of a lab patient's record for his inspection files,
the tech should: - CORRECT ANSWER: Deny the inspector a copy of the record
See HIPAA compliance policy
A tech suspects an inversion of chrom 9 with G-banding analysis. What banding
technique would confirm this? - CORRECT ANSWER: C-banding
A technologist wants to stain chromosomes using a banding technique that manifests
AT-rich regions on chromosomes. What staining procedure should he use? - CORRECT
ANSWER: QFQ
, According to CAP regulations, what is the minimum band level for consitutional
specimens? - CORRECT ANSWER: 400
The suggested level for most constitutional specimen is 550, but 400 is the lowest
acceptible level
See CAP regulation CYG.42200
After G-banded analysis, a cyto director suspects chrom 10 has a terminal del. What
banding procedure would be MOST helpful in determining if a deletion has occurred? -
CORRECT ANSWER: FISH
After performing koehler illumination, what other scope part should be adjusted to
ensure optimal contrast between light and dark bands of the chroms? - CORRECT
ANSWER: The condenser diaphragm should be closed down by 1/4 to 1/3
After staining, the chromosomes appear to have indistinct bands with little contrast. The
problem is corrected by: - CORRECT ANSWER: Increasing trypsin exposure (chroms
were muddy and dark)
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important
to monitor to assess the source of the problem? - CORRECT ANSWER: Temperature,
CO2 level, and
Humidity level
All of the following ARE characteristics of the horizontal laminar flow hood? - CORRECT
ANSWER: Protects the culture from contamination
Should not be used with transformed cell lines.
Sterlizes the air entering the hood
All of the following chromosomes have dark bands on chromosome regions that can be
used as indicators when preparing the best image: - CORRECT ANSWER:
Chromosome 11p
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