Exam (elaborations)
Microbiology Lab UTA – Final Exam Questions and Answers
- Course
- Institution
Exam of 43 pages for the course Micro UTA at Micro UTA
[Show more]
Content preview
Microbiology Lab UTA – Final ExamQuestions and Answers
How is Media made?
~~~ 5 g. T.S.A. and 125 mL Di water, cover flask with foil, affix autoclave tape,
autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or stop
when covers bottom.
Agar
~~~ linear polysaccharide polymer derived from agarose in red algae.
What does agar do?
~~~ increases solidity, exhibits hysteresis- gels and melts at different temps. Melts
above 85-90 and solidifies at 40-45.
What is liquid media?
~~~ commonly called broths, doesn't contain agar, easy to use, allows observation
of growth patterns.
Advantages to using semi-solid media
~~~ To test oxygen requirements, to test motility.
,Fastidious
~~~ only grows in a fortified culture media under specific conditions. The more
_____________ the organism, the more specific ingredients a medium must have.
Uses defined media.
Why use solid media?
~~~ The maintenance and growth of stock cultures, to isolate single colonies, to
ensure purity.
T.S.A. Plate
~~~ Tryptic Soy Agar most common medium used in lab. Undefined media that
contains casein digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of?
~~~ Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of?
~~~ 70%
Autoclave
,~~~ sterilizes with steam at 121* C, pressure at 15 PSI for at least 15 min. Tested
weekly to daily.
Reservoir
~~~ potential source of infection.
Normal commensal symbionts
~~~ S. pneumoniae and s. aureus
Environmentally acquired microbes
~~~ p. aeruginosa and b. cepacia
Magnifications of our microscopes
~~~ scanning: 4x-40, low: 10x-100, high: 40x-400, oil immersion: 100x-1000
What is resolving power?
~~~ How close two objects can be and still be distinguished as separate.
What is the optimum resolution of the microscope using oil immersion?
~~~ .2 micrometer.
, How much does each microscope cost?
~~~ $1600
convex
~~~ Morphology of a domed colony
raised
~~~ flatter dome than convex
flat elevation
~~~
crateriform
~~~ hole in convex colony
Umbonate
~~~ Morphology of a colony with a raised center
Rhizoid
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller ExamArsenal. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
82013 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now