Exam (elaborations)
Microbiology Lab UTA – Final Exam Questions and Answers
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Exam of 43 pages for the course Micro UTA at Micro UTA
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Microbiology Lab UTA – Final ExamQuestions and Answers
How is Media made?
~~~ 5 g. T.S.A. and 125 mL Di water, cover flask with foil, affix autoclave tape,
autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or stop
when covers bottom.
Agar
~~~ linear polysaccharide polymer derived from agarose in red algae.
What does agar do?
~~~ increases solidity, exhibits hysteresis- gels and melts at different temps. Melts
above 85-90 and solidifies at 40-45.
What is liquid media?
~~~ commonly called broths, doesn't contain agar, easy to use, allows observation
of growth patterns.
Advantages to using semi-solid media
~~~ To test oxygen requirements, to test motility.
,Fastidious
~~~ only grows in a fortified culture media under specific conditions. The more
_____________ the organism, the more specific ingredients a medium must have.
Uses defined media.
Why use solid media?
~~~ The maintenance and growth of stock cultures, to isolate single colonies, to
ensure purity.
T.S.A. Plate
~~~ Tryptic Soy Agar most common medium used in lab. Undefined media that
contains casein digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of?
~~~ Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of?
~~~ 70%
Autoclave
,~~~ sterilizes with steam at 121* C, pressure at 15 PSI for at least 15 min. Tested
weekly to daily.
Reservoir
~~~ potential source of infection.
Normal commensal symbionts
~~~ S. pneumoniae and s. aureus
Environmentally acquired microbes
~~~ p. aeruginosa and b. cepacia
Magnifications of our microscopes
~~~ scanning: 4x-40, low: 10x-100, high: 40x-400, oil immersion: 100x-1000
What is resolving power?
~~~ How close two objects can be and still be distinguished as separate.
What is the optimum resolution of the microscope using oil immersion?
~~~ .2 micrometer.
, How much does each microscope cost?
~~~ $1600
convex
~~~ Morphology of a domed colony
raised
~~~ flatter dome than convex
flat elevation
~~~
crateriform
~~~ hole in convex colony
Umbonate
~~~ Morphology of a colony with a raised center
Rhizoid
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