Consider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4).
Locus Tested PF Child Paternity Index
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
LOC-C3 15/17 9/17 5.21
LOC-D4 12 12 1.37
Based on the dat...
Consider the table below where a child and a possible father (PF) share the listed paternity indices for
each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4).
Locus Tested PF Child Paternity Index
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
LOC-C3 15/17 9/17 5.21
LOC-D4 12 12 1.37
Based on the data presented in the table, what is the combined paternity index, CPI, from the loci
tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4?
12.42
9.558
15.56
2.38 correct answers12.42 ( CPI is calculated by multiplying all Paternity index tgt)
Best way to avoid PCR contamination correct answersSeparate Pre-PCR (clean area) and post PCR (dirty
area)
A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA correct answersProtein
contamination . A260/280 = 1.3
<1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good RNA
Why is Fluorometry a better technique that Spectroscopy correct answersIncreased sensitivity and more
importantly can accurately measure DNA concentration of a structurally intact double stranded DNA
molecule
,Linkage Disequilibrium correct answersLD is the non random association of alleles at 2 or more loci in a
general population. When alleles are in LD, haplotypes do not occur at the expected frequencies.
Limit of Detection (LOD) correct answerslowest concentration of analyte that can be detected
mRNA Sequence provided - Convert it into DNA correct answers(Template/Anti-sense Strand) [Replace U
with T]
Correct order of HIV Sample Preparation Workflow correct answersPlasma Preparation
RNA Extraction
RNA Amplification/Modification Procedure
If a flask of cells is provided for the intended purpose of DNA isolation, how would you proceed further
correct answersTrypsinize and/or scrape the cells inside the provided flask, collect the cells suspended in
the media, spin, and then proceed further
Which end of the primer is critical for the increased specification of PCR Amplification process correct
answers3'End of the Primer
Primer Dimer Complementarity correct answers5' End of the Primers (3-3' Was not an option)
Mechanism of Action of MecA Gene in MRSA correct answersAltered Drug Target (PBP2A)
Technique to be used for FFPE Cervical Tissue Provided correct answersInvader Assay (Non-Amplification
Method)
Liquid Based-Cytology Useful for what kind of virus correct answersHPV is the correct answer (Options
Provided for: HIV, HBV, HCV, HPV)
,Isothermal Method that utilizes Primes and Polymerase to Create Synthetic dsDNA correct answersSDA
(Strand Displacement Amplification)
Polymerase used in Isothermal LAMP Procedure correct answersBST (Bacillus Stearothermophilus)
BRAF V600E Mutation is Associated with correct answersLynch Syndrome (Also Known as HNPCC)
CAP Requires Discontinued SOP/Procedures to be stored for how long correct answersAt least 2 years
from the date of discontinuation
CAP Requires Laboratory Personnel (Technologist) Proficiency Testing correct answersOnce a Year
Most Important Component of Clinical Laboratory Completed Validation Studies correct answersFinal
Test Validation Summary Signed off by Laboratory Director
STR Electropherogram provided and select the most informative loci correct answersTwo different alleles
each was both recipient vs donor the most informative one
STR makes use of which of the following correct answersVariable Nucleotide
Experiment Performed (RFLP + PCR + Gel Electrophoresis): three bands observed in the mutant sequence
as compared to the normal sequence correct answersInsertion of New Restriction Site
Mutation in what gene decreases metabolism of Irinotecan Drug correct answersUGT1A1
CYP2D6 Enzyme Metabolizes what drug correct answersCodeine
Determine Melting Temperature of a given DNA Sequence correct answers2(A+T) + 4(G+C)
Specimen Collection for Testing of Cystic Fibrosis correct answersEither 1 ml EDTA Lavender Tube/5 ml
ACD Tube (Not Sure: I think I answered 1 ml EDTA, however the volume seems to be too low)
, Fish is used in correct answersPrader Willi Syndrome
Defects associated with Prader Willi Syndrome correct answersUniparental Disomy and Genomic
Imprinting - Paternal Chromsome Deletion 15
Which of the Following is Trinucleotide Repeat Expansion Disorder correct answersHuntington's Disease,
Fragile X
Overexpression of estrogen, so treat with Herceptin/Tamoxifin Trastuzumab therapy
T-Cell Receptor Gene Rearrangement correct answersHodgkin Lymphoma (PS: Don't get confused with T-
Cell Lymphoma that corresponds to Sezary Syndrome, both options will be present for confusion)
Genes Involved in the Cell Cycle Process are termed as correct answersCycle-Dependent
What is TP53 correct answersTumor Suppressor Gene
PML/RARA Fusion occurs in which Leukemia correct answersAP (Bit Strange 2 questions on the same
leukemia)
Acute Promyleocytic Lekemia (APL) Translocation Corresponds to correct answerst(15;17)
BAM/SAM Files Purpose correct answersContains the Sequenced Aligned Data
How to run multiple unknow samples in a simple NGS Run correct answersBarcoding the Samples
Indexing is performed in NGS correct answersTo Barcode/Label/Tag DNA Sample of Interest
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