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Molecular Biology ASCP Practice Questions 2023 with correct answers

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Molecular Biology ASCP Practice Q 2023

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Molecular Biology ASCP Practice Q 2023

This refers to a means by which large quantities of DNA can be amplified from a small amount of DNA template: A. PCR B. DNA mass production C. DNA synthesis D. DNA birth correct answersPCR With regards to PCR, which of the following is NOT accurate? A. Denature strands at 94 degrees B. Bas...

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molecular biology ascp practice questions 2023

Institution Molecular Biology ASCP Practice Q 2023
Course Molecular Biology ASCP Practice Q 2023

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Molecular Biology ASCP Practice
Questions 2023

This refers to a means by which large quantities of DNA can be amplified from a small amount of DNA
template:

A. PCR

B. DNA mass production

C. DNA synthesis

D. DNA birth correct answersPCR

With regards to PCR, which of the following is NOT accurate?

A. Denature strands at 94 degrees

B. Base pairing of primers at 60 degrees

C. Elongation of primers using Taq polymerase at 72 degrees

D. Filtration of DNA at 40 degrees correct answersFiltration of DNA at 40 degrees

Which of the following PCR techniques is the best one for detecting point mutation in DNA?

A. RT PCR

B. PCR

C. Allele-specific PCR

D. Anchored PCR correct answersAllele-Specific PCR

What happens after many cycles of PCR?

A. Exponential Amplification

B. Diminished Amplification

C. Product lysing

D. DNA translation correct answersExponential Amplification

,Which of the following is NOT true about the Taq DNA polymerase?

A. It allows for the ability to use PCR to automate the process of amplification

B. It can be heat resistant

C. It is ubiquitous in nature except for the hot springs

D. It is most efficient at a certain optimal temperature. correct answersC

The PCR technique used to specifically amplify only one strand of the target DNA by using only one
unequal primer concentration is:

A. qPCR

B. Asymmetric PCR

C. Anchored PCR

D. Nested PCR correct answersAsymmetric PCR

Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100 degrees for an
hour. What is the likely result of her PCR?

A. She will get much more amplified DNA product

B. She will not get a significant amplified DNA product

C. She will only get minimal synthesis

D. She will likely not get any synthesis correct answersShe will likely not get any synthesis

When setting up her thermocycler, Sarah wants to anneal her DNA strands at a temperature much higher
than 60 degrees. What is the likely result of her action?

A. The DNA strands will likely not anneal as desired.

B. The DNA strands will likely anneal more optimally.

C. The DNA strands will anneal, and then form a triple helix.

D. The DNA strands will anneal but will not form a double helix. correct answersThe DNA strands will
likely not anneal as desired.

The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is:

A. Anchored DNA

, B. Colony PCR

C. qPCR

D. pPCR correct answersColony PCR

When setting up her thermocycler, Sarah wants to base pair her DNA strands at a temperature much
lower than 60 degrees. What is the likely result of her action?

A. She will only get single-stranded DNA products

B. The primers will likely stick to other regions of the genome

C. The primers will stick to the 5' ends only

D. The primers will stick to the 3' ends only correct answersThe primers will likely stick to other regions
of the genome.

Which of the following is true?

A. Elongation occurs at 90 degrees

B. Taq polymerase activity is near optimal at 72 degrees

C. Taq polymerase activity is near optimal at 50 degrees

D. Polymerase is not required for the elongation step of PCR correct answersTaq polymerase activity is
near optimal at 72 degrees.

This PCR variant uses degenerate primers to amplify unknown sequences of DNA, related to a known
DNA sequence is:

A. Degenerate PCR

B. Multiplex PCR

C. Hotstart PCR

D, Inverse PCR correct answersDegenerate PCR

Which of the following is true about agarose gel electrophoresis?

A. Used to separate bad proteins from good ones

B. Used to separate different DNA molecules based on their length

C. Used to lyse proteins with ethidium bromide (EtBr) straining

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