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Gel Electrophoresis – Questions With Detailed Answers
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Gel Electrophoresis – Questions With Detailed Answers
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Gel Electrophoresis – Questions With Detailed AnswersWhat is gel electrophoresis? Right Ans - A way to sort and measure DNA
strands
Why is gel electrophoresis necessary? (size) Right Ans - DNA is so small,
most microscopes can't see it
What charge is in the gel where the strands are injected? Right Ans -
Negative
What charge is in the gel opposite of where the gel is injected? Right Ans -
Positive
Why must an electrical charge be applied to the gel? Right Ans - So the DNA
can move
Which sized DNA strands show up farthest from the gel walls? Right Ans -
Short
How are the bands on the gel formed? Right Ans - During electrophoresis,
the strands move and make sorted groups
What must be done to the gel in order to see the bands on the gel? Right
Ans - The gel must be stained
What materials are needed to make a gel? Right Ans - Powdered agarose,
buffer, flask, microwave, gel mold, and comb
What is agarose? Right Ans - Dried powder similar to gelatin but made
from seaweed
What is the buffer? Right Ans - Salt water
What is the buffer used for? Right Ans - It will let the electrical charges flow
through the gel
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