Exam (elaborations)
Gel Electrophoresis – Study Set With Solutions (Rated A+)
- Course
- Institution
Gel Electrophoresis – Study Set With Solutions (Rated A+)
[Show more]
Content preview
Gel Electrophoresis – Study Set With Solutions (RatedA+)
Define Gel Electrophoresis Right Ans - laboratory method used to separate
mixtures of DNA according to molecular size. Molecules are separated by
being pushed through an electrical field through a gel that contains small
pores.
What are other techniques used to separate and identify molecules? Right
Ans - distillation, crystallization, liquid chromatography, gas chromatography,
atomic absorption
Who invented gel electrophoresis? Right Ans - Oliver Smithies
Define the process of gel electrophoresis Right Ans - applying an electrical
current to a gelatin-like substance containing biological samples. When
mixtures are placed within the wells of the gel and an electrical current is
applied , the molecules travel through the gel and separate from one another
according to each molecule's charge, size and shape.
What is the agarose made from? Right Ans - an extract of red algae
What is agarose? Right Ans - it acts as a type of molecular strainer and
prevents all the molecules from moving too quickly. The electric current
moves DNA molecules across the agarose. The agarose gel is used to visualize
the fragments. It can be used to separate DNA molecules ranging from several
hundred nucleotides in length to ober 10,000 nucleotides.
What is the function of electrophoresis buffer? Right Ans - the buffer keeps
the pH within a narrow range. The charge is also transmitted via ions
provided by the buffer.
What is the function of the wells in the gel? Right Ans - the sample being
ran in gel electrophoresis is loaded into the wells of the gel.
What is the function of the electric current? Right Ans - shocks it so that it
will move towards the positive and negative sides.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Zendaya. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75057 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now