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EXAM A-49 SUPERVION OF STORAGE HANDLING AND USE OF AEROSOL ACTUAL QUESTIONS AND ANSWERS WRITTEN AT CHAMBRLIAN COLLEGE OF NURSING AND WAS UPDATED ON JUN 27 2024$23.99
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EXAM A-49 SUPERVION OF STORAGE HANDLING AND USE OF AEROSOL ACTUAL QUESTIONS AND ANSWERS WRITTEN AT CHAMBRLIAN COLLEGE OF NURSING AND WAS UPDATED ON JUN 27 2024
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EXM A-49 SUPERVION OF STORAGE HANDLING AND USE OF
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EXM A-49 SUPERVION OF STORAGE HANDLING AND USE OF
EXAM A-49 SUPERVION OF STORAGE HANDLING
AND USE OF AEROSOL ACTUAL QUESTIONS AND
ANSWERS WRITTEN AT CHAMBRLIAN
COLLEGE OF NURSING AND WAS UPDATED ON
JUN 27 2024
What feature is commonly seen in the sequences recognized by type II restriction
enzymes? - ANS--Solution:
The recognition sequence...
EXAM A-49 SUPERVION OF STORAGE HANDLING
AND USE OF AEROSOL ACTUAL QUESTIONS AND
ANSWERS 2024-2025 WRITTEN AT CHAMBRLIAN
COLLEGE OF NURSING AND WAS UPDATED ON
JUN 27 2024
, What feature is commonly seen in the sequences recognized by type II restriction
enzymes? - ANS✔✔--Solution:
The recognition sequences are palindromic, and 4−8 base pairs long.
What normal role do restriction enzymes play in bacteria? How do bacteria protect their
own DNA from the action of restriction enzymes? - ANS✔✔--Solution:
Restriction enzymes cut foreign DNA, such as viral DNA, into fragments. Bacteria
protect their own DNA by modifying bases, usually by methylation, at the recognition
sites.
Explain how gel electrophoresis is used to separate DNA fragments of different lengths.
- ANS✔✔--Solution:
Gel electrophoresis uses an electric field to drive DNA molecules through a gel that acts
as a molecular sieve. The gel is an aqueous matrix of agarose or polyacrylamide. DNA
molecules are loaded into a slot or well at one end of the gel. When an electric field is
applied, the negatively charged DNA molecules migrate toward the positive electrode.
Shorter DNA molecules are less hindered by the agarose or polyacrylamide matrix and
migrate faster than longer DNA molecules, which must wind their way around obstacles
and through the pores in the gel matrix.
What is DNA fingerprinting? What types of sequences are examined in DNA
fingerprinting? - ANS✔✔--Solution:
DNA fingerprinting is the typing of an individual for genetic markers at highly variable
loci. This is useful for forensic investigations, to determine whether the suspect could
have contributed to the evidentiary DNA obtained from blood or other bodily fluids found
at the scene of a crime. Other applications include paternity testing and the identification
of bodily remains.
The first loci used for DNA fingerprinting were variable number of tandem repeat
(VNTR) loci; these consist of short tandem repeat sequences located in introns or
spacer regions between genes. The number of repeat sequences at the locus does not
affect the phenotype of the individual in any discernible way, so the numbers of repeats
at each of these loci are highly variable in the population. More recently, loci with
smaller repeat sequences of just a few nucleotides, called short tandem repeats
(STRs), have been adopted because they can be amplified by PCR. The variable
number of repeats creates PCR fragments of different sizes. Genotyping at 13 to 15 of
these unlinked STR loci can identify one individual among trillions of potential
genotypes.
After DNA fragments have been separated by gel electrophoresis, how can they be
visualized? - ANS✔✔--Solution:
DNA molecules can be visualized by staining with a fluorescent dye, such as ethidium
bromide, that intercalates between the stacked bases of the DNA double helix, and the
dye-DNA complex fluoresces when irradiated with an ultraviolet light source.
Alternatively, they can be visualized by attaching radioactive or chemical labels to the
DNA before it is placed in the gel.
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