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LECTURE NOTES: VIDEO PART 2 OF BIOCHEMISTRY (BIOSCI98) AT UCI
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Notes on lecture video part 2 of Biochemistry course (BIOSCI98) at University of California, Irvine. Completed between weeks 4 and 6 of Winter 2018 quarter.

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  • August 19, 2024
  • 2
  • 2018/2019
  • Other
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Part 2 - Video Lecture Notes
Thursday, February 7, 2019 11:08 AM




Separation Techniques Review


• SDS PAGE: electrophoresis gels of SDS-bound proteins


• 1 band is found on a PAGE:


○ Most likely 1 protein present, however it could also be more than one protein with the same MW.


• Isoelectric focusing:


○ All proteins first inserted into the side with high pH (basic).


○ Proteins that are at pHs higher than their pI-> negatively charged.


○ Proteins will move down the strip in order to reach their pI, @ a neutral zero charge.


• Proteome: visual of all the proteins in a cell. Similar to genomes.


• Comparing proteomes thru 2D gels can help identify differences in healthy vs. cancerous tissues. Ex. One spot is darker in gel->


protein is being up-regulated in cancerous colon tissue vs healthy colon tissue.

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