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CMB2001 L01 EXAM QUESTIONS WITH 100% CORRECT ANSWERS
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CMB2001 L01 EXAM QUESTIONS WITH 100% CORRECT ANSWERS...
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CMB2001 L01 EXAM QUESTIONS WITH 100%CORRECT ANSWERS
What is the span of the core/basal region of the eukaryotic promoter? - ANSWER
-30 to +30nt
What elements are in the core region of the eukaryotic promoter? - ANSWER
TATA box
Inr (initiator)
DPE (downstream promoter element)
BRE (TFIIB recognition element)
Describe the TATA box consensus sequence - ANSWER -31 to -26nt,
TATAWAWR
Describe the Inr sequence - ANSWER -2 to +4nt, YYANWYY with an A at +1
What is observed in core promoter CpG islands without obvious TATA and Inr
sequences? - ANSWER Lower transcription rate, initiation at multiple start sites
What is the difference between a distal and proximal regulatory promoter element?
- ANSWER Proximal = within 1kb of the start site
Distal = 1kb or more away from the start site
How do you identify promoter elements via sequence comparison? - ANSWER
Analyse base frequency within many genes to compare and identify a consensus
sequence
How do you identify promoter elements via reporter analysis? - ANSWER Insert a
reporter gene downstream of a regulatory sequence within a plasmid and measure
the expression of the reporter protein.
The impact of mutations and control, induction, timing and location of transcription
can be measured by this.
Describe RNA polymerase I - ANSWER Found in the nucleolus, it transcribes 28S,
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