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Bio205L midterm Exam | Questions And Answers Latest {} A+ Graded | 100% Verified
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Bio205L midterm Exam | Questions And Answers Latest {} A+ Graded | 100% Verified
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Bio205L midterm Exam | Questions And Answers Latest {2024- 2025} A+Graded | 100% Verified
safety guidelines in lab - - no drinking or eating
- hair tied back if long
- close toed shoes
- must be wearing lab coat, no exposed clothing or skin
- lab tables should be sanitized with 10% bleach before and after use
what is 10% bleach used for - sanitizing lab stations before and after use
ubiquity - the fact of appearing everywhere or of being very common
fomites - any substance or object that adheres to and transmits infectious material
nosocomial infection - a disease acquired in a hospital or clinical setting
bacterial colony - site of bacterial growth; cluster of microorganism growing on agar surface
bacterial culture - The growing of micro-organisms on a culture medium, such as agar, in an incubator
kept at body temperature, for purposes of identification.
turbidity - A measure of how clear water is. (cloudiness)
Why are agar media plates inverted when placed in the incubator or refrigerator? - to prevent
condensation from forming on the lid
total magnification of a microscope - Calculated by multiplying the power of the microscope's eyepiece
(10x) and the power of the objective used to view the specimen (either 4x, 10x, or 40x)
, simple vs compound microscope - Simple - only has one lens, objects are enlarged (magnified).
Compound - has two lenses and uses visible light to illuminate cells.
par focal - when the objective lens of the microscope is changed, only a minimum of refocusing is
needed
what are the 2 factors that enhance resolution - - using an objective lens with a higher numerical
opening
- using a shorter wavelength of light with immersion oil
Why is oil used with the 100X objective? - It has the same refractive properties as glass which decreases
the loss of refractive light and improves resolution
List and describe the rationale for the steps required to prepare a bacterial smear. - 1. Place a drop of
water into the wax circle that has been created on the slide.
2. Heat fix the loop and obtain a very small sample of the bacterial colony.
3. Gently mix the bacteria into the water drop.
4. Let the sample air dry.
5.heat fix the sample to kill the bacteria, firmly attach the smear to the microscope slide, allow the
sample to more readily take up the stain
Compare and contrast the staining characteristics of three species of bacteria and bacteria found in
teeth scrapings using positive and negative staining techniques. - bacillis subtilis=white
strep mutans= white
mouth organisms= white w/black ring
Gram positive: purple/blue
Gram negative: pink/red
Name four positive stains and two negatives stains and explain how their chemical charge affects
staining of bacteria. - positive: stain blue because of their thick peptidoglycan walls
- streptococcus pyogenes
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