Biosafety Levels outline what 3 things - -precautions
-special practices
-decontamination procedures
Labs are divided into how many biosafety levels - 4
(levels 1, 2, 3, and 4)
Complete information and recommendations can be found what published document and website -
Biosafety in Microbiological and Biomedical Laboratories 5th Edition
Define primary barriers and name examples - physical barriers or personal protective equipment
between lab worker and pathogen
Gloves, masks, special breathing apparatuses
Define Secondary barriers and give examples - structural aspects of the laboratory that make working
environment safer against infection
Sinks for handwashing, special containment areas, special air ventilation patterns
, Which biosafety level contains agents not known to cause disease in healthy adults but some organisms
may cause disease in immunocompromised individuals? - BSL 1
use of mechanical pipettes only (no mouth pipetting and
"sharps" precautions are standard practices for which level? - BSL 1
Examples of primary barriers for BSL 1 - -Lab Coat
-Gloves
-Face protection
-Eye protection
What are the safety practices for BSL 1? - there are none
Which level lab is suitable for work involving well characterized agents not known to cause disease in
healthy adult humans and of minimal potential hazard to laboratory personnel and the environment
(Essentially, this is open bench-top works not unlike high school science classes) - BSL 1
Which level is associated with human disease
Generally required for any human-derived blood, bodily fluids, tissues in which infectious agent may be
unknown - BSL 2
Primary hazards foe BSL 2 - Primary hazards:
accidental needle sticks
exposure to eyes and nose (mucous membranes)
ingestion of infectious material
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