Exam (elaborations)
AAB Basic Knowledge-MT Questions and Answers 2024
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AAB Basic Knowledge-MT
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AAB Basic Knowledge-MTCentrifuges (4) - answer#1- Table top centrifuges are used in clinical labs to separate
cells from serum or plasma.
#2- Micro-centrifuges are small centrifuges used for small plastic conical containers with
an integral snap cap.
#3- High speed centrifuges reach speeds b/w 17,000 & 30,000 rpm.
#4- Ultra Centrifuges are high speed centrifuges (15,000 rpm). Small air-driven to
separate chylomicrons from serum.
Pipettes types (3) - answer1. Automated pipettes
2. semi-automated micro-pipettes
3. Glass pipettes
Automated Pipettes - answerused to prep many samples for analysis and can be apart
of an automated analyzer.
Semi-automated micro-pipettes - answercontain or deliver small volumes of liquid
ranging from 1 to 1000ul.
A. air displacement- forward and reverse mode
b. positive displacement
glass pipette - answerA. transfer/ volumetric pipettes have a bulb in the middle with
tapered delivery tip.
B. measuring/ graduated pipettes are uniform in diameter with tapered delivery tip.
C. To contain (TC) or rinse out pip. must be rinsed with appropriate solvent after the
initial liquid has been drained.
D. To deliver (TD) pip are filled and allowed to drain by gravity. Most be held vertically
and top against the side of receiving vessel.
E. To deliver/ blow-out pip are filled and allowed to drain, after which the remaining fluid
in the tip is blown out. Identified by the two frosted bands near the moth piece.
Temperature-regulated equipment (4) - answerA. Heat blocks/ water baths (36C-38C)
B. Room Temperature (20C-24C)
C. Refrigerators:
Lab: 2C-8C
Blood Bank: 1C-6C
D. Freezers:
Lab: < -18C
Blood Bank: < -65C
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