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ASCP MB EXAM PRACTICE QUESTIONS

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ASCP MB EXAM PRACTICE QUESTIONS

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  • August 20, 2024
  • 25
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ASCP MB
  • ASCP MB
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GEEKA
ASCP MB EXAM PRACTICE QUESTIONS

Mutation in UGT1A1 affects metabolism of what drug?
Irontecan
Phenobarbital
Indinavir
Sorafenib
Irontecan
Its discovery shed light on why there is simultaneous, though not continuous,
synthesis of DNA on both leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments
Okazaki fragments
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt’s lymphoma
Acute promyelocytic leukemia
Ewing sarcoma
Clinical laboratories must have clearly written protocols in place describing
handling of specimens for clinical testing. Many factors can affect testing
performance before the actual testing is conducted. Such factors or variables are
collectively known as pre-analytical. From the list below, select all the pre-
analytical variables that can have a negative impact on clinical testing (Hint: more
than one answer choice!)
Storage conditions
Patient identifiers
Anticoagulant in collection tubes

,Transport procedures
Introduction of Heparin in all blood tubes to maintain specimen integrity for testing
Thermo cycling parameters
DNA isolation method used
Testing analysis software used
Storage conditions

Patient identifiers

Anticoagulant in collection tubes

Transport procedures
Imagine you are working in specimen processing for the molecular laboratory
and you receive a patient’s specimen collected in a lavender top vacutainer tube
containing EDTA. The test that is ordered requires that the specimen be collected
in a yellow top ACD vacutainer tube. What is the BEST action to take in this
instance?
Reject the specimen, inform the supervisor and request a new specimen drawn in ACD
Process the sample according to laboratory protocol because EDTA does not interfere
with molecular testing methods
Document the tube additive change, process the sample, run the test, and hold the
results until a supervisor or laboratory director signs off
Follow the prescribed laboratory protocol for accepting and rejecting specimens
Both B & D are correct
Both C & D are correct
Follow the prescribed laboratory protocol for accepting and rejecting specimens
Which one of the following complementary pairings is incorrect?
A=T
G=C
U=T
A=U
U=T

, Purines and pyrimidines differ from each other in that:
Purines are found RNA; pyrimidines are found in DNA
There’s no difference between purines and pyrimidines
Pyrimidines have two rings; purines have one ring
Purines have two rings; pyrimidines have one ring
Purines have two rings; pyrimidines have one ring
This polymerase is involved in “initiation of DNA replication and has primase
activity”:
Pol α
Pol β
Pol γ
Pol δ
Pol α
Bone marrow transplantation is used to treat several malignant and benign cancers,
particularly leukemias. Donor matching to assess immune system compatibility is a
central step of any transplantation procedure. Blood samples for both a donor and a
recipient were sent to your lab for a possible bone marrow transplantation analysis. For
a pre-transplantation assessment, your lab performed amplification of the DNA samples
(specific loci) of both donor and recipient, then resolved the DNA on polyacrylamide gel.
90 days later after transplantation (assuming donor matching was successful), a blood
specimen from the recipient was sent to your lab to assess whether or not
transplantation was successful. The gel result obtained is shown below. (Note:
R=Recipient; D=Donor; M=Molecular Weight Maker; left side of gel =pre-
transplantation; right side of gel=post-transplantation).

based on the gel image above, what can be said about the transplant procedure?
The transplant was successful!
The transplant was unsuccessful!
Not enough information provided!
The transplant was unsuccessful!

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