BIOC LAT 2 Exam Questions with 100% Complete Answers | 2024/2025 Update
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BIOC LAT
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BIOC LAT
BIOC LAT 2 Exam Questions with 100%
Complete Answers | 2024/2025 Update
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The percentage oxyhaemoglobin in a haemoglobin solution can be
calculated by:
using the ratio of absorbances of oxy- and deoxyhaemoglobin at
wavelengths where the biggest difference in absorbance r...
The percentage oxyhaemoglobin in a haemoglobin solution can be
calculated by:
using the ratio of absorbances of oxy- and deoxyhaemoglobin at
wavelengths where the biggest difference in absorbance readings are
observed between the two forms.
adding a live yeast suspension to an oxyhaemoglobin solution and observe
the change in absorbance by spectrophotometry.
measuring the absorbance of an oxyhaemoglobin solution using the
wavelength that corresponds to its absorption maxima and then apply the
Beer-Lambert formula.
,measuring the absorbance of a haemoglobin solition at 560 nm, which is
deoxyhaemoglobin's absorption maxima and the wavelenth where
oxyhaemoglobin absorb light the weakest. - ✔✔using the ratio of
absorbances of oxy- and deoxyhaemoglobin at wavelengths where the
biggest difference in absorbance readings are observed between the two
forms.
Absorbance may be defined as:
the ratio of lightpath length to incident light
the log of the ratio of incident light to transmitted light
the ratio of concentration to transmitted light
the ratio of molar absorbance coefficient to light path length - ✔✔the log of
the ratio of incident light to transmitted light
Beer's Law implies that:
the concentration of a solution is dependent on light path length
, the distance light travels through a solution is independent of concentration
the molar absorbance coefficient is dependent on the concentration of the
absorbing solute
the absorption of light increases with increasing solute concentration -
✔✔the absorption of light increases with increasing solute concentration
When haemoglobin in oxygenated in the lungs it is most likely to: - ✔✔
A 3 mL cuvette was filled with 0.5 mL of solution Y and 2.5 mL of water and
its absorbance at 340 nm was 0.394.
The molar absorbance coefficient of solution Y is 31,000 L/mol/cm. The
pathlength is 1 cm.
What is the concentration of the diluted solution Y?
Express your answer in μM and with two decimal points. - ✔✔12.71
A spectrophotometric experiment yields the following data of a 1 in 3
diluted sample:
c = 2.3 mM
A = 0.46
l = 5 mm
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