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Genetic Engineering Study Guide Test. Production of human proteins by recombinant bacteria DNA analysis to identify individuals DNA analysis to determine tissue compatibility for organ transplants Production of antiviral drugs to treat influenza - CORRECT ANSWER examples of practical app...

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  • August 22, 2024
  • 6
  • 2024/2025
  • Exam (elaborations)
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Genetic Engineering Study Guide Test.

Production of human proteins by recombinant bacteria

DNA analysis to identify individuals

DNA analysis to determine tissue compatibility for organ transplants

Production of antiviral drugs to treat influenza - CORRECT ANSWER examples of practical applications of
genetic manipulation.



True - CORRECT ANSWER T/F In the laboratory, the chemistry of DNA allows varying temperatures to be
used to separate the two stands and replicate the DNA.



Gel electrophoresis - CORRECT ANSWER Which of the following techniques utilizes an electric current
and the fact that DNA molecules are negatively charged?



Restriction enzymes are used to cut DNA at specific locations - CORRECT ANSWER Please choose the
statement that best describes the role of restriction enzymes in genetic engineering.



Southern blot analysis is used to identify DNA fragments that are carrying specific DNA sequences. -
CORRECT ANSWER Please choose the statement that best expresses the goal of Southern Blot analysis.



RNA polymerase

DNA polymerase

Gel electrophoresis

dNTP deoxynucleotide triphosphates (dATP, dTTP, dGTP,dCTP)

Primers

Fragment of DNA to be sequenced - CORRECT ANSWER Please select all of the necessary components
for sequencing DNA. Assume you have already purified the DNA that you wish to sequence.



Denaturation - CORRECT ANSWER Heating target DNA to 94 degrees Celsius to separate it into two
strands

, Priming - CORRECT ANSWER Cooling to approximately 60 degrees Celsius to allow the oligonucleotide
primers base-pair with specific sites



Extension - CORRECT ANSWER Raising the temperature to 72 degrees Celsius and adding DNA
polymerase and nucleotides



DNA (gene) to be cloned

ddNTPs

Cloning vector

Restriction endonucleases

RNA polymerase

Screening for recombinants

Bacterium serving as host (cloning host) - CORRECT ANSWER Please select all of the materials and
procedures necessary to clone a gene into a bacterium. Assume the gene to be cloned has been isolated
and purified



Antibiotics - CORRECT ANSWER Which of the following is NOT a type of protein product obtained
through recombinant DNA technology



true - CORRECT ANSWER T/F Biotechnologists have developed and tested bacteria that may be used to
clean up oil spills



false - CORRECT ANSWER T/F One benefit of transgenic animals is that they will not automatically
transcribe and translate eukaryotic genes and accurately carry out posttranslational modification of the
protein



Ti - CORRECT ANSWER A cloning vector used specifically to introduce DNA to plants is the __________
plasmid



Herbicide resistant plants

Insect resistant plants

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