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ASCP(MB) Exam Questions and Complete Solutions Graded A+
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ASCP(MB) Exam Questions and Complete Solutions Graded A+
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ASCP(MB) ExamQuestions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
, FRET - Answer: (Fluorescent Resonance Energy Transfer)
Uses TWO distinct probes to generate a signal.
probe 1: DONOR 5'
probe 2: REPORTER 3'
both anneal onto one strand of amplicon: SIGNAL.
When does a SYBR green probe generate a signal during PCR? - Answer: Extension
When does a MBG probe generate a signal during PCR? - Answer: (minor groove binder) Annealing
When does a molecular beacon probe generate a signal during PCR? - Answer: Annealing
When does a TaqMan probe generate a signal during PCR? - Answer: Extension (chomp chomp)
When does a Scorpion probe generate a signal during PCR? - Answer: Extension
Which technique employs the amplification of the signal resulting from probe:target hybridization rather
than by amplifying the target or the probe? - Answer: branched DNA analysis (BDA)
It's performed by capturing a target nucleic acid onto a microtiter plate and then adding an amplifier
probe to greatly increase the signal resulting from the captured nucleic acid.
SIGNAL AMPLIFICATION TECHNIQUES - Answer: Branched DNA analysis
Hybrid Capture Assays
Cleavage-Based Amplification
Cycling Probes
Branched DNA analysis - Answer: SIGNAL AMPLIFICATION
Capture probes immobilize target, extender probes bind to target, amplifiers give off loads of signal.
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