Exam (elaborations)
ASCP(MB) Exam Questions and Complete Solutions Graded A+
- Course
- Institution
ASCP(MB) Exam Questions and Complete Solutions Graded A+
[Show more]
Content preview
ASCP(MB) ExamQuestions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
, FRET - Answer: (Fluorescent Resonance Energy Transfer)
Uses TWO distinct probes to generate a signal.
probe 1: DONOR 5'
probe 2: REPORTER 3'
both anneal onto one strand of amplicon: SIGNAL.
When does a SYBR green probe generate a signal during PCR? - Answer: Extension
When does a MBG probe generate a signal during PCR? - Answer: (minor groove binder) Annealing
When does a molecular beacon probe generate a signal during PCR? - Answer: Annealing
When does a TaqMan probe generate a signal during PCR? - Answer: Extension (chomp chomp)
When does a Scorpion probe generate a signal during PCR? - Answer: Extension
Which technique employs the amplification of the signal resulting from probe:target hybridization rather
than by amplifying the target or the probe? - Answer: branched DNA analysis (BDA)
It's performed by capturing a target nucleic acid onto a microtiter plate and then adding an amplifier
probe to greatly increase the signal resulting from the captured nucleic acid.
SIGNAL AMPLIFICATION TECHNIQUES - Answer: Branched DNA analysis
Hybrid Capture Assays
Cleavage-Based Amplification
Cycling Probes
Branched DNA analysis - Answer: SIGNAL AMPLIFICATION
Capture probes immobilize target, extender probes bind to target, amplifiers give off loads of signal.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller YourExamplug. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $14.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
66579 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now