Exam (elaborations)
ASCP Molecular Biology Certification Exam Questions and Complete Solutions Graded A+
- Course
- Institution
ASCP Molecular Biology Certification Exam Questions and Complete Solutions Graded A+
[Show more]
Content preview
ASCP Molecular BiologyCertification Exam
Questions and Complete
Solutions Graded A+
Denning [Date] [Course title]
,Pyrimidine - Answer: One carbon ring
Cytosine, Thymine, Uracil
What is the function of mRNA? - Answer: Carries genetic info out of nucleus
Transcript translated to protein
What is the function of tRNA? - Answer: Carries aa to ribosome
Anticodon pairs with codon on mRNA strand
What is the function of rRNA? - Answer: part of ribosome structure
most abundant RNA
coordinated coupling of tRNA to mRNA codons
Feedback inhibition - Answer: Product of pathway is noncompetitive inhibitor
Binds to allosteric site to slow down rxn b/c too much product
Endonucleases (Prok) - Answer: Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Recognition site is palindromic sequence
Types I-V
ORI sites - Answer: nt sequence where replication is initiated
Topoisomerase I - Answer: Induces ss breaks
Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination; for
chr condensation; and to disentangle intertwined DNA during mitosis
topoisomerase II - Answer: cuts both strands of one DNA double helix, passes another unbroken DNA
helix through it, and then reanneals the cut strands
,Gyrase (topoisomerase II) - Answer: Unwinds supercoiling caused by unwinding at the rep fork by
introducing DSBs
Helicase - Answer: Breaks H-bonds of double helix at the replication fork
single-strand DNA binding proteins (SSBPs) - Answer: Binds ssDNA and prevents it from re-annealing
during TXN, replication, repair, and recombination
Telomeres - Answer: Repeat sequence (TTAGGG) at the ends of chr, protect chr from degradation
RNA polymerase - Answer: DNA dependent RNApol
Transcribes DNA template to RNA (3'-->5'; anti-parallel)
Splicesomes - Answer: Complex of snRNPs
Removes introns from pre-mRNA and splices exons together
Enhancers - Answer: Short regions of DNA that bind proteins (TXN factors) that enhance TXN of a gene
Reverse transcriptase - Answer: enzyme that transcribes RNA to cDNA (lacks introns)
RNA --> RNA:DNA --> cDNA (dsDNA)
Open Reading Frame (ORF) - Answer: sections of DNA that begin with start codons and end with stop
codons
DNA: 5' --> 3'
transcription: 3' --> 5' DNA --> RNA (promoter)
translation: 5' --> 3' mRNA
Spectrophotometer - Answer: Measures amount of light absorbed
Quantitative measurement of [DNA/RNA]
, At what wavelength does DNA and RNA absorb? - Answer: 260 nm
At what wavelength does protein absorb? - Answer: 280 nm
Organic isolation method - Answer: 1. Lyse
2. Add phenol/ chloroform > vortex/spin
3. Transfer aqueous layer (top) to new tube
4. Add chloroform:IAA (removes phenol) > vortex/spin
5. Transfer aqueous layer to new tube
6. Add NaOAc and EtOH > vortex/spin
7. Decant
8. Resuspend
What does the incubation step in hybridization do? - Answer: Allows formation of ds molecules
Formamide acts as a __________ in a hybridization. - Answer: denaturing agent
Line Probe Assay (LiPA) - Answer: reverse hybridization assay using sequence-specific oligonucleotide
probes (reverse SSOP)
multi-parameter testing --> single strip
Single-Stranded Conformational Polymorphism Ananlysis (SSCP) - Answer: Used for known gene,
unknown mut
Mutation screening
Short PCR products form 3D conformation when cooled --> muts have different conformation than WT
Non-denaturing PAGE, muts migrate different than WT
How does EtBr cause DNA to fluoresce? - Answer: Intercalates into the double helix
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller YourExamplug. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $14.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
81989 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now