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ASCP CG Certification Practice Exam Questions and Complete Solutions Graded A+
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ASCP CG Certification Practice Exam Questions and Complete Solutions Graded A+
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ASCP CG CertificationPractice Exam Questions
and Complete Solutions
Graded A+
Denning [Date] [Course title]
,What is the best agent to use against mycoplasma? - Answer: BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: - Answer:
comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - Answer: Proteolytic enzymes - break down tissues into single-
cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation
with: - Answer: Colcemid - mitotic spindle poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - Answer: Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? - Answer: T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is the MOST
important to document? - Answer: Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - Answer: Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? - Answer: Decidua
(maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the
mets, what is the most likely cause? - Answer: innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of cultures? - Answer:
Asceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be done next?
- Answer: prepare additional slides from any remaining sample
, When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what
could help fix the issue? - Answer: Increading the drying time by using warm slides
What is true of an inverted microscope? - Answer: the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - Answer: DiGenorge syndrome --
microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST important data to
accompany the specimen? - Answer: Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? - Answer:
Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: - Answer: Dividing
leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to monitor to
assess the source of the problem? - Answer: Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase contrast scope
is: - Answer: Slide drying after the cells have been dropped
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