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MIP 300 Final exam questions with 100% correct answers

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MIP 300 Final exam questions with 100% correct answers who provides the lipids in a viral envelope - ANSWERS the cell all viruses have a protein coat - ANSWERS true a virulent phage can inadvertently package host cell chromosome into phage particles - ANSWERS true you have a bacterium that pi...

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  • August 22, 2024
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norajuma13
MIP 300 Final exam questions with
100% correct answers

who provides the lipids in a viral envelope - ANSWERS the cell



all viruses have a protein coat - ANSWERS true



a virulent phage can inadvertently package host cell chromosome into phage particles - ANSWERS true



you have a bacterium that picks up a new gene by Hfr conjugation. what is an explanation of why
progeny of this bacterium express this trait? - ANSWERS the gene is integrated into the genome



after successful transformation of a fragment of chromosomal DNA, the recipient (female) will become
a donor (male) capable of creating a sex pili to transfer DNA - ANSWERS false



you isolate a virulent page. It can perform specialized transduction - ANSWERS false



you want to know if a baby still has vaccine neutralizing antibody that it received from its mom in utero.
Which of the following types of ELISAs would you run? one that tests for - ANSWERS IgG



in differential centrifugation, virus is first centrifuged at a slow speed, the supernatant is removed and
re-centrifuged at a very fast speed - ANSWERS true



Virus stocks prepared by differential centrifugation and gradient centrifugation are equally clean -
ANSWERS false



hemagglutination assay - ANSWERS determines highest dilution of virus that causes red blood cells to
clump together

,cytopathic effect - ANSWERS a visible effect on a host cell, caused by a virus, that may result in host cell
damage or death



immuno fluorescence assay - ANSWERS primarily on biological samples and is classically defined as a
procedure to detect antigens in cellular contexts using antibodies.



plaque assay - ANSWERS method used to measure the number of viral particles present in a sample



a temperate phage can inadvertently package host cell chromosome into phage particles - ANSWERS
true



you isolate a bacterium that can transfer genes to other bacteria only when it is in direct contact with
these bacteria. it is most likely transferring the genes by - ANSWERS conjugation



a piece of host cell dna bound to a piece of phage dna after excising from the chromosome. when this
phage that contains host cell dna infects a new cell, will the new cell be killed by this infection? -
ANSWERS no



after successful generalized transduction, the recipient (female) will become a donor (male) capable of
creating a sex pili to transfer dna - ANSWERS false



can a temperate phage perform generalized and specialized transduction? - ANSWERS yes



what kind of organism can carry out the conversion of O2 --> H2O? - ANSWERS aerobic respiration and
aerobic chemolithotrophy



an error in dna replication causes a mutation changing AGG to CGG, what change do you expect? -
ANSWERS no change to protein function



the organism with the more positive reduction potential will produce more energy - ANSWERS true

, dna ligase forms - ANSWERS the last phosphodiester bond between dna single strands



dna ligase forms hydrogen bonds - ANSWERS false



for each acetyl-coA that enters the TCA cycle, how many ATP are generayed by substrate level
phosphorylation - ANSWERS 1



you correctly perform a gran stain on bacteria that contain LPS, what color will you see on the slide? -
ANSWERS pink



a bacterium contains 2 carbohydrates and 5 different amino acids in its cell wall. What color will it gram
stain? - ANSWERS purple



an antibiotic inhibits ribosome function and prevents bacteria from sporulating. which step in
sporulation is affected - ANSWERS formation of spore coats



a bacterium is known to be a psychophile. which of the following would be true for this organism? -
ANSWERS generation time shortest at 0-20 degrees celcsius and growth rate the fastest



you grow a bacteria in a tube that is loosely capped. it grows evenly throughout the tube. the bacteria is
likely - ANSWERS aerotolerant anaerobe



a bacterium has recently acquired antibiotic resistance, which of the following could NOT be a way this
occurred - ANSWERS someone stopped taking her antibiotics early



which of the following antibiotic mechanisms would be achieve selective toxicity - ANSWERS stop cell
wall synthesis



what is the most effective type of vaccine? will you have full immunity if you get the vaccine 5 days
before exposure - ANSWERS attenuated; no

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