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MCB 2610 Lab Practical (Questions & Answers) Rated 100%
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Gram positive bacteria do not retain CV when gram stained, so they appear pink after counter-staining with safranin. T/F - False. They do retain crystal violet and appear purple Complex media is made with precise chemical composition. T/F - False. Complex media has unknown composition ______ is...

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  • August 23, 2024
  • 6
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • MCB 2610 Lab Practical
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MCB 2610 Lab Practical
Gram positive bacteria do not retain CV when gram stained, so they appear pink after counter-staining
with safranin. T/F - False. They do retain crystal violet and appear purple



Complex media is made with precise chemical composition. T/F - False. Complex media has
unknown composition



______ is an opportunistic pathogen that causes staph infections. - Staphylococcus aureus



A durham tube is included in the LT broth tubes to detect _________, a byproduct of lactose
fermentation - gas production



Brownian motion is the purposeful movement of microorganisms. T/F - False. It is random
vibrations of organisms coming into contact with water molecules



Cells that do not retain crystal violet and appear pink are said to be - Gram negative



Cells that retain crystal violet and appear purple are - Gram positive



Coliforms are a subgroup of enteric bacteria classified by their ability to ferment which sugar? -
Lactose



Define generation time - The amount of time it takes a population to double



Eosin-methylene blue (EMB) agar is used as a confirmed test for coliforms. T/F - True



Fomite examples - cell phone, table, door knob

, Generation time is calculated in log phase. T/F - True



Gram positive bacteria have a thick peptidoglycan layer which retains safranin and appears pink. T/F -
False. Retains crystal violet and appears purple.



Gram staining steps - 1. apply bacterial sample to glass slide

2. heat fix slide

3. crystal violet solution (1 min)

4. rinse with water

5. gram's iodine (mordant for CV) (1 min)

6. rinse

7. decolorizer (alcohol wash) (10 sec)

8. rinse

9. safranin (counter stain) 1 min

10. rinse with water and pat dry



gram (+) : purple

gram (-) : pink



Gram stains and spore stains are examples of simple stains. T/F - False. These are differential
staining techniques



Heat staining causes sporulation. T/F - False.



How is casein hydrolysis detected? - Clearing zone around colonies



How many times do you get culture from the tube while performing a T streak? - 1



Is LT broth selective, differential or both? - Both

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