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Unit 2 Microbiology Lab Exam Questions and Complete Solutions Graded A+ $14.49   Add to cart

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Unit 2 Microbiology Lab Exam Questions and Complete Solutions Graded A+

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  • Course
  • Microbiology 2104
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  • Microbiology 2104

Unit 2 Microbiology Lab Exam Questions and Complete Solutions Graded A+

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  • August 26, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Microbiology 2104
  • Microbiology 2104
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Unit 2 Microbiology
Lab Exam
Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]

, Recombination is referring to combining two sources of: - Answer: dna



The enzyme which cuts apart DNA is called: - Answer: restriction endonuclease



In the simulation that you performed at home, how did you simulate the enzyme which cuts apart DNA?
- Answer: scissors



The enzyme which was used to cut the DNA was used to cut: - Answer: the human insulin gene out of
the human genome

open the plasmid



Could a different enzyme be used to cut out the human gene than was used to open up the plasmid? -
Answer: no



The restriction enzyme you used was called EcoRI. What does Eco stand for? - Answer: Escherichia coli



What did the tape simulate? - Answer: The bonding between the ends of the plasmid to the ends of the
human insulin gene

an action of the enzyme called ligase



The circular white strip of paper represented a: - Answer: bacterial plasmid



The blue strip of paper represented the: - Answer: human insulin gene



T/F To be able to connect the ends of the plasmid to the ends of the human insulin gene, you needed to
use a restriction endonuclease which made a staggered cut along the double stranded DNA. This would
allow the single stranded ends to overlap. Moreover, the base pairing would have to follow the rule of A
with T and G with C. - Answer: true



What medium do you use for a throat culture? - Answer: blood agar

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