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Bio 2104 Lab Practical Final Exam Questions and Complete Solutions Graded A+
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Bio 2104 Lab Practical Final Exam Questions and Complete Solutions Graded A+
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Bio 2104 LabPractical Final
Exam Questions
and Complete
Solutions Graded
Denning [Date] [Course title]
, course adjustment knob - Answer: Moves the stage up and down for focusing
fine adjustment knob - Answer: Fine tunes the focus and increases the detail of the specimen.
oil immersion lens (100x) - Answer: only lens that uses oil
labeling plates - Answer: -Label bottom (agar side) of the plate
- Do not label lid
-Plates should be inverted when incubating
streak plate technique - Answer: a technique which produces isolated colonies of an organism
gram positive stain - Answer: retain crystal violet and stain purple, thick peptidoglycan cell wall
gram negative stain - Answer: pink or red, thin peptidoglycan and outer membrane
endospore stain - Answer: Used to distinguish endospores from vegetative cells. Endospores appear
green
capsule staining - Answer: Negative stain - capsules colorless against a stained background
acid fast stain - Answer: Acid fastness is a physical property that gives a bacterium the ability to resist
decolorization by acids during staining procedures. used to detect TB and mycobacterium
acid fast bacteria - Answer: Retain the primary stain even when treated with acid alcohol. (pink)
serial dilution - Answer: Dilution of a substance several times by the same amount each time to produce
a countable (between 30 and 300 colonies)
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